Thermo Fisher Scientific IL-4 Polyclonal Antibody, PeproTech
Rat IL-4 단백질을 인식하는 Rabbit Polyclonal 항체로, Western Blot, ELISA, Neutralization 등에 사용 가능. 항원 친화 크로마토그래피로 정제되었으며, PBS 내 동결건조 형태로 제공. 연구용으로만 사용.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.1–0.2 µg/mL | – |
| ELISA (ELISA) | 0.5–2.0 µg/mL | – |
| Neutralization (Neu) | – | 1 publication |
| Miscellaneous PubMed (Misc) | – | 1 publication |
| Immunostaining (IS) | – | 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Rat |
| Published Species | Rat |
| Host/Isotype | Rabbit |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | E.coli-derived Recombinant Rat IL-4 |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.1–1.0 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient |
| RRID | AB_2930869 |
Product Specific Information
AA Sequence of recombinant protein:
MHGCNDSPLR EIINTLNQVT EKGTPCTEMF VPDVLTATRN TTENELICRA SRVLRKFYFP RDVPPCLKNK SGVLGELRKL CRGVSGLNSL RSCTVNESTL TTLKDFLESL KSILRGKYLQ SCTSMS.
Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Rat IL-4. Anti-Rat IL-4-specific antibody was purified by affinity chromatography employing an immobilized Rat IL-4 matrix.
Sandwich ELISA:
To detect Rat IL-4 by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.5–2.0 µg/mL of this antibody is required. This affinity-purified antibody, in conjunction with PeproTech Biotinylated Anti-Rat IL-4 (500-P94Bt) as a detection antibody, allows detection of at least 0.2–0.4 ng/well of Recombinant Rat IL-4.
Western Blot:
To detect Rat IL-4 by Western Blot analysis, use at 0.1–0.2 µg/mL. With compatible secondary reagents, the detection limit for Recombinant Rat IL-4 is 1.5–3.0 ng/lane under reducing or non-reducing conditions.
Target Information
Interleukin-4 (IL-4) is a pleiotropic, immune-modulatory cytokine produced by activated T cells and serves as a ligand for the interleukin 4 receptor. IL-4 exhibits a broad range of biological actions and shares overlapping functions with IL-13. STAT6 plays a key role in mediating the IL-4 signaling pathway. The IL-4 gene is located near IL-13 within a cytokine gene cluster on chromosome 5q, coordinated by long-range regulatory elements across approximately 120 kb. IL-4 delta 2 is a known splice variant.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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