Thermo Fisher Scientific BCKDH Polyclonal Antibody, FITC
FITC로 표지된 BCKDH 폴리클로날 항체로, Western blot, ELISA, IP에 사용 가능. 인간, 생쥐, 랫트 반응성. 합성 펩타이드 면역원 사용. 액상 형태로 -20°C 암소 보관. 연구용으로만 사용.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| ELISA | 1:10,000 |
| Immunoprecipitation (IP) | 1:50–1:250 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide within amino acid region 170–200 of human BCKD protein |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 0.5–1.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | Proprietary buffer, pH 7.4–7.8, with 30% glycerol, 0.5% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, store in dark |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
The branched-chain alpha-keto acid dehydrogenase complex (BCKD) catalyzes the second major step in the catabolism of branched-chain amino acids (isoleucine, leucine, and valine).
This inner-mitochondrial enzyme complex includes three catalytic components:
- E1: Branched-chain alpha-keto acid decarboxylase
- E2: Dihydrolipoyl transacylase
- E3: Dihydrolipoamide dehydrogenase
Additionally, two regulatory enzymes—BCKDK (kinase) and a phosphorylase—control the activity state of the BCKD complex.
Defects in this pathway can cause Maple Syrup Urine Disease (MSUD), leading to accumulation of branched-chain amino acids and neurodegeneration in untreated infants.
For Research Use Only.
Not for use in diagnostic procedures or resale without express authorization.
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