Thermo Fisher Scientific BID Monoclonal Antibody (23F7)
Human 및 Mouse에서 BID 단백질을 검출하는 23F7 클론 단일클론 항체. Western blot과 ICC/IF에 최적화되어 있으며, Protein A로 정제된 액상 형태. PBS/BSA 완충액에 보존되며 -20°C에서 안정적으로 보관 가능.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Mouse / IgG |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 23F7 |
| Immunogen | Residues 5–195 from human recombinant BID protein expressed in bacteria |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS with 1 mg/mL BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_10985736 |
Product Specific Information
MA1-13043 detects BID from human and mouse samples.
It has been successfully used in ICC/IF and Western blot procedures.
By Western blot, this antibody detects a 22 kDa protein corresponding to human BID.
The MA1-13043 antigen is a recombinant protein corresponding to residues 5–195 of human BID.
Target Information
BID is a death agonist that heterodimerizes with either agonist BAX or antagonist BCL2.
It is a member of the BCL-2 family of cell death regulators and mediates mitochondrial damage induced by caspase-8 (CASP8).
CASP8 cleaves BID, and the COOH-terminal fragment translocates to mitochondria, triggering cytochrome c release and apoptosis.
BID serves as a direct molecular link between caspase-8 activation and mitochondrial death machinery.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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