Thermo Fisher Scientific 6x-His Tag Monoclonal Antibody (4E3D10H2/E3)
6x-His 태그 단백질 검출용 Thermo Fisher Scientific 단클론 항체. Western blot, ELISA, IP 등 다양한 응용 가능. N- 및 C-말단 His-tag 인식. 높은 특이성과 재현성을 제공하며, Protein A 정제 및 안정적 저장 가능.
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Thermo Fisher Scientific 6x-His Tag Monoclonal Antibody (4E3D10H2/E3)
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:250–1:1,000 | 19 |
| Immunohistochemistry (Paraffin) (IHC (P)) | – | 1 |
| Immunocytochemistry (ICC/IF) | 5 µg/mL | 1 |
| Flow Cytometry (Flow) | – | 2 |
| ELISA | 25–500 ng/mL | 5 |
| Immunoprecipitation (IP) | 2 µg | 5 |
| Dot Blot (DB) | – | 1 |
| Miscellaneous PubMed (Misc) | – | 2 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Tag |
| Published Species | Tag |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 4E3D10H2/E3 |
| Immunogen | Mixture of N-terminal and C-terminal 6x-His peptides (CG-HHHHHH and HHHHHH-GC) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS with 1 mg/mL BSA, 30% glycerol |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C (short term); for long-term, store at −20°C avoiding freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2536841 |
Additional Formats
- Alexa Fluor 488 (MA1-135-A488)
- Alexa Fluor 555 (MA1-135-A555)
- Alexa Fluor 647 (MA1-135-A647)
- HRP (MA1-135-HRP)
- Request custom conjugation
Product Specific Information
By Western blot, MA1-135 detects both N-terminal and C-terminal His-tagged proteins.
Target Information
The 6x His tag is a synthetic oligopeptide consisting of six consecutive histidine residues (HHHHHH). It is commonly expressed at either the N- or C-terminal regions of recombinant proteins to facilitate purification via immobilized metal affinity chromatography (IMAC).
Epitope tagging enables detection of recombinant proteins even when specific antibodies are unavailable. His-tagged proteins bind strongly and selectively to Ni²⁺ ions, allowing purification to homogeneity using Ni²⁺-NTA adsorbents.
His tag-specific antibodies are used for detection or co-immunoprecipitation of His-tagged proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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