Thermo Fisher Scientific CD10 Chimeric Recombinant Rabbit Monoclonal Antibody (NL-1)

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

-

Immunocytochemistry (ICC/IF)

10 µg/mL

-

Immunoprecipitation (IP)

Assay-dependent

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG, kappa

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

NL-1

Immunogen

BALB/c mice were immunised with acute lymphocytic leukemia cells from a human leukemia patient. Splenocytes were obtained from immunised mice and fused with MOPC-21 NS/1 myeloma cells to generate a hybridoma. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:CD10,uniProtId:P08473-1,ncbiNodeId:9606,antigenRange:1-750,antigenLength:750,antigenImageFileName:MA5-48117_CD10_P08473-1_Rabbit.svg,antigenImageFileNamePDP:MA5-48117_CD10_P08473-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

0.02% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_3074560

Product Specific Information

Specificity: This antibody reacts with human CD10. CD10 is a widely-expressed cell-surface metalloprotease, responsible for the cleavage of multiple peptide hormones at target tissues. CD10 is also a common marker for acute lymphocytic leukaemia.

Target Information

CD10 (Common Acute Lymphocytic Leukemia Antigen, CALLA), is a cell surface enzyme with neutral metalloendopeptidase activity which inactivates a variety of biologically active peptides. CD10 is expressed on the cells of lymphoblastic, Burkitts, and follicular germinal center lymphomas, immature B cells with in adult bone marrow and on cells from patients with chronic myelocytic leukemia (CML). CD10 is also present on breast myoepithelial cells, bile canaliculi, fibroblasts, with especially high expression on the brush border of kidney and gut epithelial cells. CD10 is a neutral endopeptidase that cleaves peptides at the amino side of hydrophobic residues and inactivates several peptide hormones including glucagon, enkephalins, substance P, neurotensin, oxytocin, and bradykinin. Further, CD10 is a 100 kDa type II transmembrane glycoprotein that exists in a single copy of greater than 45 kb. The 5 untranslated region of the CD10 gene is alternatively spliced, resulting in four separate mRNA transcripts and the coding region is not affected by alternative splicing. Diseases associated with CD10 dysfunction include spinocerebellar ataxia 43 and Charcot-Marie tooth Disease.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.