Thermo Fisher Scientific alpha-1 Antitrypsin Monoclonal Antibody (2B12)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 2 publications 2 publications

ELISA (ELISA)

1:10,000

-

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

2B12

Immunogen

Purified recombinant fragment of human AAT expressed in E. Coli.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

ascites

Contains

0.03% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_10985373

Product Specific Information

MA5-15521 targets AAT in indirect ELISA, WB applications and shows reactivity with Human and mouse samples.

The MA5-15521 immunogen is purified recombinant fragment of human AAT expressed in E. Coli.

MA5-15521 detects AAT which has a predicted molecular weight of approximately 47kDa.

Target Information

Alpha-1-AT is synthesized in the liver and it acts as an inhibitor of proteases such as trypsin, elastase, chymotrypsin, collagenase, leucocytic proteases, plasmin, and thrombin, which may be released during inflammatory reactions in the lung. In the absence of alpha-1-AT, these enzymes are not inhibited and they may digest pulmonary parenchyma. Alpha-1-AT deficiency is associated with chronic obstructive lung disease (emphysema) and less frequently with hepatic cirrhosis in infants and respiratory distress of the newborn. Increase in alpha-1-AT occurs as an acute phase response to tissue necrosis and inflammation. Serum level of alpha-1-AT is elevated in rheumatoid arthritis, bacterial infections, vasculitis, and carcinomatosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.