Thermo Fisher Scientific Ly-108 Monoclonal Antibody (eBio13G3-19D (13G3-19D)), Functional Grade, eBioscience

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-Dependent

-

Flow Cytometry (Flow)

0.5 µg/test

-

Immunoprecipitation (IP)

Assay-Dependent

-

Functional Assay (Functional)

Assay-Dependent

-

Blocking Assay (BLOCK)

-

View 1 publication 1 publication

Product Specifications

Published species

Mouse

Host/Isotype

Mouse / IgG2a, kappa

Recommended Isotype Control

Mouse IgG2a kappa Isotype Control (eBM2a), Functional Grade, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

eBio13G3-19D (13G3-19D)

Conjugate

Functional Grade Functional Grade Functional Grade

View additional formats

• Unconjugated
• APC
• Biotin
• NovaFluor Blue 660-120S
• NovaFluor Red 685
• NovaFluor Red 700
• NovaFluor Red 755
• NovaFluor Yellow 610
• NovaFluor Yellow 660
• NovaFluor Yellow 700
• NovaFluor Yellow 755
• PE
• Request custom conjugation

Form

Liquid

Concentration

1 mg/mL

Storage conditions

4° C

Shipping conditions

Wet ice

RRID

AB_2865885

Product Specific Information

Description: The eBio13G3-19D monoclonal antibody reacts with mouse Ly108, the mouse homologue of human SLAMF6 (signaling lymphocyte activation molecule family member 6). Mouse Ly108 is also known as NTB-A (NK cell, T cell, B cell antigen), and is expressed on NK, T cells, and B cells. Engagement of the Ly108 receptor enhances NK cell activity and expression of IFN-gamma, and contributes to the inability of NK cells to kill Epstein Barr virus-infected B cells in X-linked lymphoproliferative disease. CD4+ T cells from Ly108 knock-out mice exhibit diminished ability to produce IL-4 and defective neutrophil functions.

Staining has not revealed any strain differences between C57BL/6 and BALB/c mice. Other strains have not been tested.

Applications Reported: This eBio13G3-19D (13G3-19D) antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunoblotting (WB).

Applications Tested: This eBio13G3-19D (13G3-19D) antibody has been tested by flow cytometric analysis of mouse splenocytes and thymocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Storage and handling: Use in a sterile environment.

Filtration: 0.2 µm post-manufacturing filtered.

Purity: Greater than 90%, as determined by SDS-PAGE.

Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.

Aggregation: Less than 10%, as determined by HPLC.

Target Information

Ly108 is the mouse homologue of human SLAMF6 (signaling lymphocyte activation molecule family member 6). Mouse Ly108 is expressed on NK, B cells, and T cells. Activation of Ly108 receptor enhances NK cell activity and IFN-gamma expression.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.