Thermo Fisher Scientific VISTA Monoclonal Antibody (MIH64), Super Bright 436, eBioscience

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

1 µg/test

-

Product Specifications

Species Reactivity

Mouse

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), Super Bright™ 436, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

MIH64

Conjugate

Super Bright™ 436 Super Bright™ 436 Super Bright™ 436

View additional formats

• APC
• Brilliant UV 615
• Brilliant UV 737
• NovaFluor Blue 660-120S
• NovaFluor Yellow 610
• NovaFluor Yellow 730
• PE
• PerCP-eFluor 710
• Super Bright 600
• Request custom conjugation

Excitation/Emission Max

413/431 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2725661

Product Specific Information

Description: This MIH64 monoclonal antibody recognizes mouse VISTA also known as B7-H5 or PD-H1.

This MIH64 antibody will work in flow cytometry on both native and paraformaldehyde-fixed cells.

Applications Reported: This MIH64 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This MIH64 antibody has been tested by flow cytometric analysis of mouse splenocytes. This may be used at less than or equal to 1 µg/mL per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Super Bright 436 can be excited with the violet laser line (405 nm) and emits at 436 nm. We recommend using a 450/50 bandpass filter, or equivalent. Please make sure that your instrument is capable of detecting this fluorochrome.

When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information.

Excitation: 405 nm; Emission: 436 nm; Laser: Violet Laser

Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

Target Information

VISTA (V-domain Ig containing suppressor of T-cell activation), also known as B7-H5 or PD-1H, is a product of the VSIR gene. Although VISTA bears sequence homology to PD-L1 it has a different expression pattern. In both mouse and humans, VISTA is expressed predominantly on hematopoietic cells with the highest densities on myeloid cells, and lower levels on T cells. It is usually absent on B cells. VISTA expressed by antigen presenting cells has been shown to directly suppress proliferation and cytokine production of CD4 and CD8 T cells, making it a target for cancer immunotherapy. The reported ligands of VISTA include CD28H, VSIG-3 and VSIG-8.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.