Thermo Fisher Scientific VISTA Monoclonal Antibody (MIH64), Super Bright 436, eBioscience
마우스 VISTA 단백질을 인식하는 MIH64 단클론 항체로, Super Bright 436 형광체가 결합되어 있습니다. 유세포 분석(Flow Cytometry)에 최적화되어 있으며, 고순도 친화 크로마토그래피 정제 제품입니다. 405 nm 레이저에 의해 여기되어 436 nm에서 방출되며, 암 연구 및 면역학 연구용으로 적합합니다.
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Thermo Fisher Scientific VISTA Monoclonal Antibody (MIH64), Super Bright 436, eBioscience
Applications
- Flow Cytometry (Flow)
Tested Dilution: 1 µg/test
Publications: -
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), Super Bright™ 436, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | MIH64 |
| Conjugate | Super Bright™ 436 |
| Excitation / Emission Max | 413 / 431 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2725661 |
Additional Formats
- APC
- Brilliant UV 615
- Brilliant UV 737
- NovaFluor Blue 660-120S
- NovaFluor Yellow 610
- NovaFluor Yellow 730
- PE
- PerCP-eFluor 710
- Super Bright 600
- Request custom conjugation
Product Specific Information
This MIH64 monoclonal antibody recognizes mouse VISTA, also known as B7-H5 or PD-H1.
It is validated for flow cytometry on both native and paraformaldehyde-fixed cells.
Applications Reported: Flow cytometric analysis
Applications Tested: Mouse splenocytes, ≤1 µg/mL per test (100 µL final volume).
Cell number may vary between 10⁵–10⁸ cells/test. Antibody titration is recommended for optimal results.
Super Bright 436 is excited by the violet laser line (405 nm) and emits at 436 nm.
Recommended filter: 450/50 bandpass or equivalent.
Ensure instrument compatibility with this fluorochrome.
When using multiple Super Bright dye-conjugated antibodies, use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize non-specific polymer interactions.
Excitation: 405 nm
Emission: 436 nm
Laser: Violet
Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
Target Information
VISTA (V-domain Ig containing suppressor of T-cell activation), also known as B7-H5 or PD-1H, is encoded by the VSIR gene.
Although homologous to PD-L1, VISTA has a distinct expression pattern—predominantly on hematopoietic cells, especially myeloid cells, and at lower levels on T cells. It is typically absent on B cells.
VISTA expressed by antigen-presenting cells can directly suppress CD4 and CD8 T-cell proliferation and cytokine production, making it a promising target for cancer immunotherapy.
Reported ligands include CD28H, VSIG-3, and VSIG-8.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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