Thermo Fisher Scientific ATF1 Monoclonal Antibody (OTI5D10), TrueMAB
ATF1 단백질을 인식하는 마우스 단클론 항체로 Western blot 및 IHC에 적합. 인간 시료 반응성, 고순도 친화 크로마토그래피 정제. 동결건조 형태로 제공되며 재구성 후 1 mg/mL 농도 유지. 연구용으로 세포 성장 및 전사 조절 연구에 활용 가능.
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Thermo Fisher Scientific ATF1 Monoclonal Antibody (OTI5D10), TrueMAB
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI5D10 |
| Immunogen | Full length human recombinant protein of human ATF1 produced in E.coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step.
Recommended desalting product: Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
The ATF1 gene encodes an activating transcription factor belonging to the ATF subfamily and bZIP (basic-region leucine zipper) family.
It regulates downstream target genes related to growth, survival, and other cellular processes.
Phosphorylation at serine 63 by various kinases enhances transactivation and transcriptional activity, contributing to cell transformation.
Gene fusion events with FUS (chromosome 16) or EWSR1 (chromosome 22) result in chimeric proteins associated with angiomatoid fibrous histiocytoma and clear cell sarcoma.
A pseudogene is present on chromosome 6.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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