Thermo Fisher Scientific CD83 Monoclonal Antibody (HB15e), FITC, eBioscience
인체 CD83 단백질을 인식하는 HB15e 클론 단일클론 항체로, FITC 형광 표지되어 있습니다. 성숙 수지상세포 표지에 유용하며, 유세포분석(Flow Cytometry)에 최적화되어 있습니다. 488 nm 레이저에서 여기, 520 nm에서 방출됩니다.
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Applications
- Flow Cytometry (Flow): 5 µL (1 µg)/test
- Tested and pre-titrated for flow cytometric analysis of normal human peripheral blood cells
- A test is defined as the amount (µg) of antibody to stain a cell sample in 100 µL final volume
- Recommended cell count: 10⁵–10⁸ cells/test
- Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser
- Filtration: 0.2 µm post-manufacturing filtered
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), FITC, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | HB15e |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 5 µL/test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_1633400 |
Additional Formats
- Unconjugated
- Alexa Fluor 700
- APC
- APC-eFluor 780
- PE
- PE-Cyanine7
- PerCP-eFluor 710
- Super Bright 436
- Super Bright 702
- Request custom conjugation available
Product Specific Information
The HB15e monoclonal antibody reacts with human CD83, a 45 kDa transmembrane glycoprotein and a member of the Ig superfamily. CD83 is expressed on cultured dendritic cells, interdigitating, follicular, and circulating dendritic cells, as well as some proliferating lymphocytes. It serves as a useful marker for mature human blood dendritic cells.
- Applications Reported: Flow cytometric analysis
- Applications Tested: Pre-titrated for flow cytometry at 5 µL (1 µg) per test
Target Information
CD83 is a 40–45 kDa glycoprotein expressed by peripheral blood dendritic cells. Peripheral lymphocytes can express low levels of CD83 after stimulation with Con A or PHA. In immunohistology, CD83 is strongly expressed by interfollicular interdigitating reticulum cells and weakly by germinal center cells. It is also expressed by Langerhans cells in the skin.
The CD83 antigen is a 186-amino-acid single-chain glycoprotein, a member of the immunoglobulin superfamily, comprising:
- Extracellular V-type Ig-like domain
- Transmembrane region
- Short cytoplasmic tail (~40 amino acids)
CD83 undergoes extensive post-translational glycosylation, resulting in a molecular weight approximately twice that of the predicted core protein. CD83⁺ cells display a unique immunophenotype distinct from T, B, NK, or myelomonocytic cells and coexpress high levels of MHC class II molecules along with markers such as CD2, CD5, CD40, CD78, CD13, CD33, and CD36.
Associated Diseases: Plague, Rift Valley Fever.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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