Thermo Fisher Scientific Ki-67 Monoclonal Antibody (20Raj1), PE-Cyanine7, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 5 µL (0.125 µg)/test
• Publications: View 5 publications

Product Specifications

Additional Available Formats

• Unconjugated
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 395 / 563 / 615 / 737 / 805
• Brilliant Violet 421 / 480 / 605 / 650 / 711 / 786
• eFluor 450 / 506 / 570 / 615 / 660
• FITC
• PE
• PE-Cyanine5.5
• PE-eFluor 610
• PerCP-eFluor 710
• Request custom conjugation

Product Specific Information

The monoclonal antibody 20Raj1 recognizes the human and canine Ki-67 protein, which exists in two isoforms (345 and 395 kDa) expressed in dividing cells. Ki-67 is present during active phases of the cell cycle (G1, S, G2, M) but absent in resting cells (G0).
During interphase, Ki-67 localizes to the nucleus and redistributes to chromosomes during mitosis, associating with heterochromatin-bound proteins such as CBX3.
In tumor studies, Ki-67 serves as a marker for the fraction of proliferating cells.

• Applications Reported: Intracellular staining followed by flow cytometric analysis
• Applications Tested: Pre-titrated and validated using the Foxp3/Transcription Factor Staining Buffer Set (Product #00-5523-00)
• Recommended Protocol: Refer to BestProtocols® - Staining intracellular antigens for Flow Cytometry (Protocol B: One-step protocol for intracellular (nuclear) proteins)

Usage: 5 µL (0.125 µg) per test (100 µL final volume).
Cell number: 10⁵–10⁸ cells/test (empirically determined).

Light Sensitivity: Protect from light to prevent photo-induced oxidation.
Fixation: Samples can be stored in IC Fixation Buffer (Product #00-8222-49) or 1-step Fix/Lyse Solution (Product #00-5333-54) for up to 3 days at 4°C in the dark.
Excitation: 488–561 nm
Emission: 775 nm
Laser Compatibility: Blue, Green, Yellow-Green
Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Ki-67 is a nuclear protein expressed during late G1, S, G2, and M phases of the cell cycle. It associates with euchromatin and heterochromatin regions and shows a cell cycle-dependent distribution pattern.
Ki-67 is widely used as a cell proliferation marker, as it is absent in G0 cells and increases progressively through mitosis.
In neoplastic tissues, Ki-67 index correlates strongly with tumor grade and is comparable in prognostic value to the thymidine-labeling index. It is also used as a neuronal proliferation marker.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

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