Thermo Fisher Scientific Ki-67 Monoclonal Antibody (SolA15), PE-Cyanine7, eBioscience
Ki-67 단백질을 인식하는 SolA15 클론의 단일클론 항체로, PE-Cyanine7 형광체가 결합되어 있습니다. 세포 증식 분석 및 종양 연구에 활용되며, 유세포분석(Flow Cytometry)에 적합합니다. 다양한 종(사람, 생쥐, 개 등)에 반응하며, 광민감성이 있어 암실 보관이 필요합니다.
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2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific Ki-67 Monoclonal Antibody (SolA15), PE-Cyanine7, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Flow Cytometry (Flow) | 0.125 µg/test | View 37 publications |
| Functional Assay (Functional) | - | View 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Dog, Cynomolgus monkey, Human, Mouse, Non-human primate, Rat |
| Published Species | Human, Mouse |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), PE-Cyanine7, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | SolA15 |
| Conjugate | PE-Cyanine7 |
| Excitation / Emission Max | 569 / 780 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_11217689 |
Available Formats
- Unconjugated
- Alexa Fluor 488, 532, 660, 700, Plus 594
- APC, APC-eFluor 780
- Biotin
- Brilliant UV 395–805
- Brilliant Violet 421–786
- eFluor 450, 506, 570, 615, 660
- FITC, PE, PE-Cyanine5, PE-eFluor 610, PerCP-eFluor 710
- Request custom conjugation
Product Specific Information
The monoclonal antibody SolA15 recognizes mouse and rat Ki-67, a 300 kDa nuclear protein expressed during all active phases of the cell cycle (G1, S, G2, M) but absent in resting cells (G0). Ki-67 is used as a marker for determining the growth fraction of cell populations and has prognostic value in cancer studies.
SolA15 also reacts with human, non-human primate, and canine Ki-67.
- Applications Reported: Intracellular staining followed by flow cytometric analysis
- Applications Tested: Tested using the Foxp3/Transcription Factor Staining Buffer Set (Product #00-5523-00)
- Recommended ≤0.125 µg/test
- A test = antibody amount for 100 µL final volume (10⁵–10⁸ cells/test)
- Optimize titration for best performance
Light Sensitivity: Protect from light; tandem dye sensitive to photo-induced oxidation.
Fixation: Samples can be stored up to 3 days in the dark at 4°C using IC Fixation Buffer (#00-8222) or 1-step Fix/Lyse Solution (#00-5333).
Excitation/Emission: 488–561 nm / 775 nm (Blue, Green, Yellow-Green Laser).
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
Ki-67 is a nuclear protein expressed during late G1, S, G2, and M phases of the cell cycle. It associates with euchromatin and heterochromatin through its FHA domain and shows cell cycle-dependent localization. Ki-67 is widely used as a proliferation marker since it is absent in G0 cells and increases through mitosis. It is valuable in evaluating tumor growth rates and prognosis, correlating with tumor grade in neoplastic tissues.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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