Thermo Fisher Scientific Ki-67 Monoclonal Antibody (SolA15), PE-Cyanine7, eBioscience

Applications and Tested Dilution

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 488, 532, 660, 700, Plus 594
• APC, APC-eFluor 780
• Biotin
• Brilliant UV 395–805
• Brilliant Violet 421–786
• eFluor 450, 506, 570, 615, 660
• FITC, PE, PE-Cyanine5, PE-eFluor 610, PerCP-eFluor 710
• Request custom conjugation

Product Specific Information

The monoclonal antibody SolA15 recognizes mouse and rat Ki-67, a 300 kDa nuclear protein expressed during all active phases of the cell cycle (G1, S, G2, M) but absent in resting cells (G0). Ki-67 is used as a marker for determining the growth fraction of cell populations and has prognostic value in cancer studies.

SolA15 also reacts with human, non-human primate, and canine Ki-67.

• Applications Reported: Intracellular staining followed by flow cytometric analysis
• Applications Tested: Tested using the Foxp3/Transcription Factor Staining Buffer Set (Product #00-5523-00)
  • Recommended ≤0.125 µg/test
  • A test = antibody amount for 100 µL final volume (10⁵–10⁸ cells/test)
  • Optimize titration for best performance

Light Sensitivity: Protect from light; tandem dye sensitive to photo-induced oxidation.
Fixation: Samples can be stored up to 3 days in the dark at 4°C using IC Fixation Buffer (#00-8222) or 1-step Fix/Lyse Solution (#00-5333).
Excitation/Emission: 488–561 nm / 775 nm (Blue, Green, Yellow-Green Laser).
Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Ki-67 is a nuclear protein expressed during late G1, S, G2, and M phases of the cell cycle. It associates with euchromatin and heterochromatin through its FHA domain and shows cell cycle-dependent localization. Ki-67 is widely used as a proliferation marker since it is absent in G0 cells and increases through mitosis. It is valuable in evaluating tumor growth rates and prognosis, correlating with tumor grade in neoplastic tissues.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

제품 이미지