Thermo Fisher Scientific Alpha A Crystallin Polyclonal Antibody
Alpha A Crystallin 단백질을 인식하는 Rabbit Polyclonal Antibody로, WB 및 ICC/IF에 적합합니다. Human, Mouse, Rat, Zebrafish 반응성을 가지며, 항원 친화 크로마토그래피로 정제되었습니다. 1 mg/mL 농도의 액상 형태로 제공되며, 4°C 또는 -20°C에서 보관합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat, Zebrafish |
| Host/Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant full length Human CRYAA (Alpha A Crystallin, UniProt ID: P02489-1, Antigen range: 1–173) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage buffer | PBS, pH 7.2, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping conditions | Wet ice |
| RRID | AB_2720533 |
Product Specific Information
The antibody was affinity-purified from rabbit antiserum by affinity chromatography using an epitope-specific immunogen. The purity is greater than 95% as determined by SDS-PAGE.
Target Information
Lens proteins consist almost entirely of crystallins (about 95%), which play a structural role in maintaining the proper refractive index of the lens. The mammalian lens contains three major classes of crystallins: alpha, beta, and gamma.
Alpha-crystallin is the largest and is composed of two primary gene products, alpha-A and alpha-B. Alpha-crystallin comprises about 40% of the total lens protein and also functions as a molecular chaperone, preventing protein aggregation that can lead to cataract formation.
Phosphorylated states of alpha-crystallin may occur in response to cellular stress and play a role in structural control and protein maintenance. Alpha-B crystallin has been linked to Alexander’s disease, where it accumulates in brain cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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