Thermo Fisher Scientific 53BP1 Polyclonal Antibody, DyLight 650
DyLight 650 형광 표지된 53BP1 폴리클로날 항체로, Western blot, IHC, ICC/IF 등 다양한 응용에 사용 가능. 인간 및 마우스 시료에서 검증됨. DNA 손상 반응 및 종양 억제 관련 연구에 적합. 항원 친화 크로마토그래피로 정제된 고품질 항체.
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Thermo Fisher Scientific 53BP1 Polyclonal Antibody, DyLight 650
Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:200–1:10,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-Dependent |
| Immunohistochemistry (Frozen) (IHC (F)) | Assay-Dependent |
| Immunocytochemistry (ICC/IF) | 1:200–1:1,000 |
| Flow Cytometry (Flow) | Assay-Dependent |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Bat, Bovine, Dog, Human, Mouse, Rodent, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Epitope maps to residues 1925–1972 of human tumor protein p53 binding protein 1 (entry NP_005648.1) |
| Conjugate | DyLight™ 650 |
| Excitation / Emission Max | 651 / 673 nm |
| Form | Liquid |
| Concentration | 0.8 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | 50 mM sodium borate |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C, store in dark |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_11153828 |
Product Specific Information
- Human samples tested in Western Blot and ICC/IF.
- Mouse samples tested in ICC/IF only.
- Suggested positive controls: U205, 293T, MO59K, and MEF cell lysates.
Target Information
53BP1 (P53-binding protein 1) plays a critical role in tumor suppression and is a substrate of ATM kinase. Upon DNA damage, 53BP1 is phosphorylated and localizes to double-strand break sites. It participates in checkpoint signaling during mitosis, enhances TP53-mediated transcriptional activation, and contributes to DNA repair and genomic stability.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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