Thermo Fisher Scientific Malondialdehyde Monoclonal Antibody (11E3)
Malondialdehyde 단백질 결합체를 특이적으로 인식하는 Mouse IgG1 단클론 항체. Western blot, ICC/IF, ELISA에 적합하며, free Malondialdehyde에는 반응하지 않음. -20°C 보관, Protein G 정제, PBS/glycerol buffer 구성.
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Thermo Fisher Scientific Malondialdehyde Monoclonal Antibody (11E3)
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000 | View 1 publication |
| Immunocytochemistry (ICC/IF) | 1:50 | - |
| ELISA | 1:1,000 | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Chemical |
| Published Species | Chemical |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 11E3 |
| Immunogen | Synthetic Malondialdehyde modified Keyhole Limpet Hemocyanin (KLH) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.09% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2735263 |
Additional Formats
Product Specific Information
A 1:1,000 dilution of MA5-27560 was sufficient for detection of Malondialdehyde in 2 µg of Malondialdehyde conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary antibody.
Specific for Malondialdehyde conjugated proteins. Does not detect free Malondialdehyde.
Does not cross-react with Acrolein, Crotonaldehyde, Hexanoyl Lysine, 4-Hydroxy-2-hexenal, 4-Hydroxy nonenal, or Methylglyoxal modified proteins.
Target Information
Malondialdehyde (CH₂(CHO)₂) is a byproduct of lipid metabolism and a highly reactive electrophile species that induces toxic stress in cells. It forms covalent protein adducts known as advanced lipoxidation end products (ALE).
It also forms mutagenic DNA adducts when reacting with deoxyadenosine and deoxyguanosine.
Malondialdehyde is found in many foods and is present in high levels in rancid foods.
Usage Note
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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