Thermo Fisher Scientific CD185 (CXCR5) Monoclonal Antibody (MU5UBEE), NovaFluor Blue 610-70S, eBioscience

Applications

• Flow Cytometry (Flow): Tested at 5 µL (1.0 µg)/test

Product Specifications

Additional Available Formats

• Unconjugated
• Alexa Fluor 532, 700
• APC, Biotin
• Brilliant UV 395, 615, 737, 805
• Brilliant Violet 421, 480, 605, 786
• eFluor 450, FITC
• NovaFluor Blue 660-120S, 725, 760, 800
• NovaFluor Red 685, 710
• NovaFluor UV 765
• NovaFluor Violet 690, 800
• NovaFluor Yellow 610, 690, 730, 810
• PE, PE-Cyanine5, PE-Cyanine7, PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436, 600, 645, 702

Product Specific Information

The MU5UBEE monoclonal antibody reacts with human and non-human primate CD185 (CXCR5/BLR1), a seven-transmembrane G protein-coupled receptor originally identified in Burkitt’s lymphoma.
In peripheral blood, CD185 is expressed on B cells, CD4⁺ T cells (excluding Th1/Th2), and a subset of memory (CD45RO⁺) T cells. It plays a key role in lymphocyte trafficking and T cell migration into B cell follicles in response to CXCL13, serving as a marker of follicular helper T cells.
This antibody cross-reacts with Rhesus macaque.

Each product includes:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-titrated for flow cytometry using normal human peripheral blood cells. Recommended use: 5 µL (0.125 µg) per test in 100 µL volume.

Note:

• NovaFluor dyes are not compatible with DNA intercalating viability dyes (e.g., propidium iodide, 7-AAD, DAPI).
• Use Invitrogen LIVE/DEAD Fixable Dead Cell stains for viability.
• CellBlox Plus Blocking Buffer (Cat. No. C001T06F01) is required for optimal staining.
  • Add 5 µL per sample to antibody cocktails before combining with cells.
  • For single-color controls: 5 µL per 100 µL cell sample (10³–10⁸ cells).

NovaFluor conjugates utilize Phiton™ technology, based on novel nucleic acid dye structures engineered for optimized fluorescence with minimal spillover and spread.

• Excitation: 509 nm
• Emission: 614 nm
• Laser: 488 nm (Blue)

Learn more about NovaFluor dyes

Target Information

CXCR5 (C-X-C Chemokine Receptor Type 5, CD185), also known as Burkitt lymphoma receptor 1 (BLR1), is a G protein-coupled receptor for chemokine BLC.
It is expressed in Burkitt’s lymphoma and lymphatic tissues such as lymph node follicles and spleen, playing a key role in B cell migration.
CXCR5 expression has been reported in human blood, salivary gland, and various animal tissues (lymph node, spleen, brain, liver, etc.).
As a member of the GPCR family, CXCR5 shares the seven-transmembrane structure typical of this receptor class, mediating signal transduction via G-proteins.
GPCRs are major pharmacological targets, with clinically significant drugs such as Zyprexa and Claritin developed against this receptor family.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.