Thermo Fisher Scientific CD223 (LAG-3) Monoclonal Antibody (3DS223H), PE-eFluor 610, eBioscience

Thermo Fisher Scientific CD223 (LAG-3) Monoclonal Antibody (3DS223H), PE-eFluor 610, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry
• Tested Dilution: 5 µL (0.125 µg)/test
• Publications: View 8 publications

Product Specifications

Additional Formats Available

• Alexa Fluor 647, 660, 700
• APC, APC-eFluor 780
• Brilliant UV/BV series (395–805)
• eFluor 450, 506
• FITC
• NovaFluor Blue/Red/Yellow series
• PE, PE-Cyanine5, PE-Cyanine7
• PerCP-eFluor 710
• Super Bright series (436–780)
• Request custom conjugation

Product Specific Information

This 3DS223H monoclonal antibody recognizes human CD223 (LAG-3), a 70-kDa surface glycoprotein belonging to the Ig superfamily with homology to CD4. LAG-3 binds MHC class II with higher affinity than CD4 and negatively regulates T cell activation and proliferation. It is expressed on activated T cells (including regulatory T cells) and NK cells, with CD8+ T cells typically showing higher expression levels.

• Epitope Recognition: Formaldehyde-fixed epitope
• Applications Reported: Flow cytometric analysis
• Applications Tested: Pre-titrated; tested on stimulated human peripheral blood cells
• Recommended Usage: 5 µL (0.125 µg) per test (100 µL final volume; 10⁵–10⁸ cells/test)

Fluorophore Information:
PE-eFluor® 610 is excited at 488–561 nm and emits at 607 nm. Recommended band pass filter: 610/20 (PE-Texas Red® equivalent).

Light Sensitivity: Protect vial and samples from light.
Fixation: Samples can be stored in IC Fixation Buffer (Product #00-8222-49) or 1-step Fix/Lyse Solution (Product #00-5333-57) up to 3 days at 4°C in the dark.
Filtration: 0.2 µm post-manufacturing filtered.

Excitation: 488–561 nm
Emission: 607 nm
Laser Compatibility: Blue, Green, Yellow-Green lasers

Target Information

LAG-3 is a 70-kDa surface glycoprotein in the Ig superfamily with homology to CD4. It binds MHC class II with high affinity and is involved in negative regulation of T cell activation and homeostatic proliferation. Expressed on activated T cells (including regulatory T cells) and NK cells; CD8+ T cells express higher levels than CD4+ T cells. Coexpression of LAG-3 and CD49b identifies Type 1 regulatory T cells (Tr1).

For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.

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