Thermo Fisher Scientific CD223 (LAG-3) Monoclonal Antibody (3DS223H), PE-eFluor 610, eBioscience
인간 CD223(LAG-3)을 인식하는 3DS223H 단클론 항체로, PE-eFluor 610 형광체가 결합된 제품입니다. 활성화된 T세포 및 NK세포 분석에 적합하며, 유세포분석용으로 최적화되어 있습니다. 488–561 nm 레이저에 의해 여기되며 607 nm에서 방출됩니다.
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Thermo Fisher Scientific CD223 (LAG-3) Monoclonal Antibody (3DS223H), PE-eFluor 610, eBioscience
Applications and Tested Dilution
- Application: Flow Cytometry
- Tested Dilution: 5 µL (0.125 µg)/test
- Publications: View 8 publications
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Not Applicable |
| Host / Isotype | Mouse / IgG1, kappa |
| Recommended Isotype Control | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE-eFluor™ 610, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 3DS223H |
| Immunogen | hCD223-FC PFA 4% |
| Conjugate | PE-eFluor™ 610 |
| Excitation/Emission Max | 565/606 nm |
| Form | Liquid |
| Concentration | 5 µL/Test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 0.2% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2574595 |
Additional Formats Available
- Alexa Fluor 647, 660, 700
- APC, APC-eFluor 780
- Brilliant UV/BV series (395–805)
- eFluor 450, 506
- FITC
- NovaFluor Blue/Red/Yellow series
- PE, PE-Cyanine5, PE-Cyanine7
- PerCP-eFluor 710
- Super Bright series (436–780)
- Request custom conjugation
Product Specific Information
This 3DS223H monoclonal antibody recognizes human CD223 (LAG-3), a 70-kDa surface glycoprotein belonging to the Ig superfamily with homology to CD4. LAG-3 binds MHC class II with higher affinity than CD4 and negatively regulates T cell activation and proliferation. It is expressed on activated T cells (including regulatory T cells) and NK cells, with CD8+ T cells typically showing higher expression levels.
- Epitope Recognition: Formaldehyde-fixed epitope
- Applications Reported: Flow cytometric analysis
- Applications Tested: Pre-titrated; tested on stimulated human peripheral blood cells
- Recommended Usage: 5 µL (0.125 µg) per test (100 µL final volume; 10⁵–10⁸ cells/test)
Fluorophore Information:
PE-eFluor® 610 is excited at 488–561 nm and emits at 607 nm. Recommended band pass filter: 610/20 (PE-Texas Red® equivalent).
Light Sensitivity: Protect vial and samples from light.
Fixation: Samples can be stored in IC Fixation Buffer (Product #00-8222-49) or 1-step Fix/Lyse Solution (Product #00-5333-57) up to 3 days at 4°C in the dark.
Filtration: 0.2 µm post-manufacturing filtered.
Excitation: 488–561 nm
Emission: 607 nm
Laser Compatibility: Blue, Green, Yellow-Green lasers
Target Information
LAG-3 is a 70-kDa surface glycoprotein in the Ig superfamily with homology to CD4. It binds MHC class II with high affinity and is involved in negative regulation of T cell activation and homeostatic proliferation. Expressed on activated T cells (including regulatory T cells) and NK cells; CD8+ T cells express higher levels than CD4+ T cells. Coexpression of LAG-3 and CD49b identifies Type 1 regulatory T cells (Tr1).
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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