Thermo Fisher Scientific BMI1 Monoclonal Antibody (BMI1, 2823)
BMI1 단백질을 인식하는 Thermo Fisher Scientific의 mouse monoclonal antibody로, WB, IHC, ICC 등 다양한 응용에 사용 가능. 인간 및 생쥐 반응성, Protein A/G 정제, PBS buffer에 보존. 연구용으로만 사용.
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Applications 및 Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| Peptide Array (Array) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | BMI1, 2823 |
| Immunogen | Recombinant fragment (aa 142–326) of human BMI1 protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | −20°C or −80°C if preferred |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Immunohistochemistry (PFA fixed): incubate antibody for 30 min at room temperature.
Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C, followed by cooling at room temperature for 20 minutes.
Target Information
BMI1 is a component of the Polycomb group (PcG) multiprotein PRC1 complex, which maintains transcriptional repression of many genes, including Hox genes, throughout development.
The PRC1 complex mediates chromatin remodeling and histone modification, specifically monoubiquitination of histone H2A at Lys-119, resulting in heritable changes in chromatin expressibility.
Within the PRC1 complex, BMI1 is required to stimulate the E3 ubiquitin-protein ligase activity of RNF2/RING2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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