Thermo Fisher Scientific RIPK3 Monoclonal Antibody (OTI3A3), TrueMAB
RIPK3 단백질을 인식하는 Mouse IgG2b 단클론 항체로, Western blot 및 IHC(P) 실험에 적합합니다. 인간 RIPK3(aa 233-518) 재조합 단백질을 면역원으로 제작되었으며, 동결건조 형태로 제공됩니다. 연구용으로만 사용 가능합니다.
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Applications
Western Blot (WB)
- Tested Dilution: 1:200–1:1,000
Immunohistochemistry (Paraffin) (IHC (P))
- Tested Dilution: 1:150
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI3A3 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 233–518 of human RIPK3 produced in E. coli |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step using Zeba Spin Desalting Columns (7K MWCO, 0.5 mL, Product #89882).
Target Information
RIPK3 is a member of the receptor-interacting protein (RIP) family of serine/threonine kinases, with a unique C-terminal domain distinct from other RIP family members. It is mainly localized in the cytoplasm and mitochondria, capable of nucleocytoplasmic shuttling via nuclear localization and export signals.
RIPK3 participates in TNF receptor-I signaling, can induce apoptosis, and weakly activates NF-κB. Caspase-8 prevents RIPK3-dependent necrosis by forming a proteolytically active complex with CFLAR, essential for protective function.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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