
Thermo Fisher Scientific NOTCH1 Monoclonal Antibody (N253/32), PerCP
NOTCH1 단백질을 인식하는 Mouse monoclonal antibody (Clone N253/32)로, PerCP 형광 표지된 제품입니다. Western blot 및 Immunocytochemistry에 사용 가능하며, 사람, 생쥐, 랫트 시료에 반응합니다. 단백질 G 정제, 보존제 무첨가, 4°C 보관.
- 카탈로그번호
- MA545606
- 판매단위
- pk
카탈로그
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunocytochemistry (ICC/IF) | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | N253/32 |
| Immunogen | Fusion protein amino acids 20–216 (extracellular N-terminus, EGF-like domains 1–5) of mouse Notch1 |
| Conjugate | PerCP |
| Excitation / Emission Max | 482 / 675 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 95.64 mM phosphate / 2.48 mM MES, pH 7.4, with 0.5 M EDTA |
| Contains | No preservative |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2932060 |
Additional Formats
Product Specific Information
1 µg/mL of MA5-45606 was sufficient for detection of Notch1 in 20 µg of rat brain membrane lysate and assayed by colorimetric immunoblot analysis using goat anti-mouse IgG:HRP as the secondary antibody.
Detects >270 kDa. Does not cross-react with Notch2 or Notch3.
This antibody was formerly sold as clone S253-32.
Target Information
The Notch gene family encodes transmembrane proteins containing multiple cysteine-rich EGF-like repeats in the extracellular region.
In mammals, four Notch genes (Notch1, Notch2, Notch3, Notch4/Int-3) are known and are involved in the differentiation of the nervous system and other structures.
EGF-like proteins Delta and Serrate act as ligands for Notch1.
Mature Notch proteins are heterodimers formed by cleavage into an extracellular subunit (NEC) and a transmembrane subunit (Ntm).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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