Thermo Fisher Scientific CD73 Monoclonal Antibody (eBioTY/11.8 (TY/11.8)), NovaFluor Yellow 755, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 0.2 µg/test

Product Specifications

Available Formats

• Unconjugated
• eFluor 450
• Functional Grade
• NovaFluor Blue 760
• NovaFluor Red 685
• NovaFluor Red 725
• NovaFluor Red 755
• NovaFluor Yellow 610
• NovaFluor Yellow 690
• NovaFluor Yellow 730
• PE
• PE-Cyanine7
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 702
• Custom conjugation available

Product Specific Information

Description:
eBioTY/11.8 recognizes CD73, a 69-kDa GPI-anchored cell-surface protein with ecto-5'-nucleotidase activity. Expression on myeloid cells (CD11b) is restricted to the bone marrow. In humans, CD73 can be induced to secrete a soluble form with IL-2, suggesting a role in activation signaling. Differences between human and mouse CD73 expression have been reported.

CD73 is expressed on a subset of lymphocytes and increases during lymphocyte maturation. It is found on memory CD4 T cells and regulatory T cells, playing a role in differentiation into Th1 or Th2 cells.

Each product contains:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Applications Reported: Flow cytometric analysis
Applications Tested: Tested by flow cytometric analysis of mouse splenocytes at ≤0.2 µg/test (100 µL final volume; 10⁵–10⁸ cells/test). Titrate for optimal performance.

Important Notes:

• NovaFluor dyes are not compatible with DNA intercalating viability dyes (e.g., propidium iodide, 7-AAD, DAPI).
• Use Invitrogen LIVE/DEAD Fixable Dead Cell stains for compatibility.
• CellBlox Plus Blocking Buffer (Cat. No. C001T06F01) must be used for optimal staining. Add 5 µL per sample to antibody cocktails or single-color controls.
• NovaFluor conjugates utilize Phiton™ technology for engineered fluorescent signatures minimizing spillover and spread.
• Excitation: 552 nm; Emission: 690 nm; Laser: 561 nm (Yellow).
• Internal testing shows non-specific B cell staining in SJL mice; not observed in BALB/c or C57BL/6 strains.

Target Information

CD73 (Ecto-5'-nucleotidase) catalyzes conversion of purine 5'-mononucleotides to nucleosides (preferentially AMP). It is a dimeric, GPI-anchored 70 kDa protein used as a marker of lymphocyte differentiation. CD73 deficiency is associated with immunodeficiency disorders. The gene is located on 6q14–q21, and defects can lead to calcification of joints/arteries and intestinal tuberculosis. Two transcript variants encoding different isoforms are known.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

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