Thermo Fisher Scientific GITRL Polyclonal Antibody

Applications and Tested Dilution

Product Specifications

Product Specific Information

• In direct ELISAs, less than 5% cross-reactivity with recombinant human APRIL, BAFF, CD27 Ligand, CD30 Ligand, CD40 Ligand, Fas Ligand, TNF-alpha, TRAIL, TRANCE, and recombinant mouse GITR Ligand is observed.
• Reconstitute at 0.2 mg/mL in sterile PBS.
• Endotoxin level: <0.10 EU per 1 µg of antibody (LAL method).

Target Information

The tumor necrosis factor (TNF) and TNF receptor (TNFR) gene superfamilies regulate various biological processes including cell proliferation, differentiation, and survival through activation of NF-kappa-B and MAP kinases.
GITR (Glucocorticoid-Induced TNF Receptor) is expressed on CD4+ CD25+ regulatory T cells and plays a key role in immune regulation. Its ligand, GITRL, is expressed in endothelial and antigen-presenting cells, activating NF-kappa-B, modulating apoptotic pathways, inhibiting regulatory T cell suppression, and co-stimulating responder T cells.
Dominant negative forms of NIK and TRAF2 expressed in transfected 293 cells inhibit NF-kappa-B activation, indicating that the GITRL–GITR pathway involves both NIK and TRAF2.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.