Norgen Plant microRNA Purification Kit

Norgen Plant microRNA Purification Kit

SKU: 54700
For the rapid purification of microRNA from plants

Features and Benefits

• Fast and easy processing using a rapid spin-column format
• No phenol or chloroform extractions
• Isolate all small RNA molecules (<200 nt)
• Minimal contamination from large RNA molecules and genomic DNA
• High quality small RNA suitable for various downstream applications
• Purification based on spin column chromatography using Norgen’s proprietary resin matrix

Product Description

Norgen’s Plant microRNA Purification Kit provides a rapid method for isolating and purifying small RNA molecules (<200 nt) from cultured plant cells or plant tissues. These include regulatory RNAs such as microRNA (miRNA) and short interfering RNA (siRNA), as well as tRNA and 5S rRNA.
Small RNAs are key regulators of gene expression, typically 20–25 nucleotides long, binding to mRNA to affect stability or translation.
Purified small RNAs can be used for RT-PCR, miRNA sequencing, northern blotting, and microarray analysis.

Supporting Data

Figure 1. Fractionation of Large and Small RNA

Large RNA and small RNA were sequentially purified using Norgen’s Plant miRNA Purification Kit from 50 mg of raspberry leaf tissue. The RNA profile was compared with RNA isolated using Norgen’s Plant/Fungi Total RNA Purification Kit (Cat. #25800).
10 µL of RNA from a 50 µL elution was loaded on a 2% agarose gel. Norgen’s kit successfully isolated both large and small RNA fractions, with small RNA fraction free of large RNA species.

이미지 OCR 텍스트:
Plant Total RNA
Plant miRNA

Figure 2. Efficiency of miRNA Concentration

Small RNA was concentrated from 50 mg raspberry using Norgen’s Plant miRNA Purification Kit. 3 µL of elution was used for cDNA generation, followed by qPCR detection using Bio-Rad CFX96.
Blue: small RNA from Norgen kit, Red: total RNA from Plant Total RNA Purification Kit (Cat. #25800).
Circle: +RT, Cross: -RT.

이미지 OCR 텍스트:
각 그래프는 다음 miRNA를 나타냅니다.

• miRNA156a
• miRNA159a
• miRNA166a
  공통 축: X축(Cycles), Y축(RFU), 제목(Amplification)

Figure 3. Isolation of Concentrated RNA for Sensitive Applications

Three different miRNA purification methods were compared for their ability to isolate and detect plant miRNA from grape samples. Only the miRNA purified using Norgen’s kit (blue lines) was amplified, while competitor kits failed due to phenol interference.
Blue: Norgen Plant miRNA, Red: Competitor Q, Green: Trizol.

이미지 OCR 텍스트:

Kit Specifications

Storage Conditions and Product Stability

All solutions should be tightly sealed and stored at room temperature.
This kit is stable for 1 year after the date of shipment.

Kit Components

Documentation

Protocols

• Plant microRNA Purification Kit - Protocol (25 prep)

Application Notes

• Evaluation of Plant RNA Integrity Number (RIN) using Agilent Bioanalyzer 2100
• Optimizing Bead Homogenization of Plant Tissues for DNA and RNA Isolation

Safety Data Sheets

• 54700 - Plant microRNA Purification Kit - SDS

Posters

• The Importance of Sample Preparation for Plant miRNA Purification

Flyers

• Plant microRNA Purification Kit Flyer

Related Blogs

• 3 Reasons why Norgen’s RNA Purification Technology is Best-in-Class
• Reducing False Negative SARS-CoV-2 Results with Silicon Carbide Technology

Citations

Related Products

Plant RNA/DNA Purification Kit

Plant/Fungi Total RNA Purification Kit

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