Invitrogen™

CellEvent™ Caspase-3/7 Green Flow Cytometry Assay Kit

The CellEvent™ Caspase-3/7 Green Flow Cytometry Assay Kit enables the flow cytometric detection of activated caspase-3 and caspase-7 in apoptotic자세히 알아보기

The CellEvent™ Caspase-3/7 Green Flow Cytometry Assay Kit enables the flow cytometric detection of activated caspase-3 and caspase-7 in apoptotic cells. The kit includes the novel fluorogenic substrate CellEvent™ Caspase-3/7 Green Detection Reagent, as well as SYTOX™ AADvanced™ Dead Cell Stain.

• Caspase-3/7 specific—includes the recognition sequence for activated caspase-3 and caspase-7
• Easy identification—clearly identify live, dead, and apoptotic cell populations
• Quick analysis—washing and fixation is not required
• Multicolor compatibility—combine with other dyes excitable by the 488 nm laser or other lasers

CellEvent™ Caspase-3/7 Green Detection Reagent is a cell-permeant reagent that consists of a four-amino acid peptide (DEVD) conjugated to a nucleic acid-binding dye. During apoptosis, caspase-3 and caspase-7 proteins are activated and able to cleave the caspase 3/7 recognition sequence encoded in the DEVD peptide. Cleavage of the recognition sequence and binding of DNA by the reagent labels the apoptotic cells with a bright, fluorogenic signal that has absorption/emission maxima of ∼511/533 nm. When used together with the SYTOX™ AADvanced™ Dead Cell Stain, apoptotic cells can be easily discriminated from live and necrotic cells.

Because no single parameter defines apoptosis in all systems, we strongly suggest using a combination of different measurements for reliable detection of apoptosis.

사양

수량100 Assays

형식Kit

제품 유형Caspase Assay Kit

유세포분석기 레이저 라인488

여기/방출SYTOX AADvanced: 546/647
CellEvent Caspase 3/7 Green Detection Reagent: 511/533

배송 조건Wet Ice

용해도DMSO (Dimethylsulfoxide)

제품라인CellEvent™

검출 방법Fluorescence

용도(애플리케이션)Flow Cytometry

용도(장비)Attune™ Acoustic Focusing Cytometer, Flow Cytometer

라벨 또는 염료CellEvent™ Caspase 3/7 Green Detection Reagent, SYTOX AADvanced dead cell stain

라벨 유형Other Label(s) or Dye(s)

Unit SizeEach