Thermo Fisher Scientific Click-iT EdU Alexa Fluor 488 Flow Cytometry Assay Kit, 100 assays

Invitrogen™ Click-iT™ EdU Alexa Fluor™ 488 Flow Cytometry Assay Kit

The Click-iT™ EdU Alexa Fluor™ 488 Flow Cytometry Assay Kit provides a simplified, robust assay for analyzing DNA replication in proliferating cells, offering superior performance compared to traditional BrdU methods. Newly synthesized DNA can be analyzed using the 488 nm laser of a flow cytometer.

• Accurate — Superior results compared to BrdU assays
• Fast — Results in as little as 90 minutes
• Economical — More assays per kit

View selection guide for all Click-iT™ EdU and Click-iT™ Plus EdU assays for flow cytometry

An Advanced Method Giving You Results Superior to BrdU

The most accurate method of proliferation analysis is direct measurement of DNA synthesis. Traditionally, this was performed using radioactive nucleosides such as ³H-thymidine, later replaced by antibody-based detection of bromodeoxyuridine (BrdU).
The Click-iT™ EdU Flow Cytometry Assay Kits are advanced alternatives using EdU (5-ethynyl-2´-deoxyuridine), a thymidine analog incorporated into DNA during synthesis. Detection is based on click chemistry — a copper-catalyzed covalent reaction between an azide and an alkyne. The alkyne is present in EdU, while the azide is coupled to Alexa Fluor™ 488, Alexa Fluor™ 647, or Pacific Blue™ dyes. Standard flow cytometry methods determine the percentage of S-phase cells in a population.

Mild Conditions Allow Use with Cell Cycle Dyes and Antibodies

The small dye azide enables efficient EdU detection under mild conditions. Standard aldehyde-based fixation and detergent permeabilization allow the Click-iT™ detection reagent to access DNA without harsh denaturation steps required for BrdU assays (e.g., HCl, heat, or DNase).
This gentle processing preserves cell cycle distribution and antigen recognition, making the EdU assay compatible with cell cycle dyes and multiplexing with antibodies against surface or intracellular markers.

Quick and Simple Protocol

The Click-iT™ EdU protocol follows standard immunolabeling fixation and permeabilization steps and is compatible with saponin and methanol.
Five simple steps to analyze cell proliferation data:

1. Treat cells with EdU
2. Fix and permeabilize cells
3. Detect S-phase cells with Click-iT™ detection cocktail (30 min)
4. Wash once
5. Analyze by flow cytometry

Get Accurate Results Economically

By enabling more assays per kit, the Click-iT™ EdU Alexa Fluor™ 488 Flow Cytometry Assay Kit provides cost-effective, high-quality results—ideal for large-scale experiments.

For Research Use Only. Not intended for any human or animal therapeutic or diagnostic use.

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