Thermo Fisher Scientific F4/80 Monoclonal Antibody (CI:A3-1)

Applications

Tested Dilution

Publications

Western Blot (WB)

10 µg/mL

View 2 publications 2 publications

Immunohistochemistry (IHC)

-

View 16 publications 16 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:100

View 1 publication 1 publication

Immunohistochemistry (PFA fixed) (IHC (PFA))

-

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

1:1,000

-

Immunocytochemistry (ICC/IF)

1:100

View 4 publications 4 publications

Flow Cytometry (Flow)

1:50-1:100

View 17 publications 17 publications

Immunoprecipitation (IP)

Assay-dependent

-

Radioimmune Assays (RIA)

Assay-dependent

-

Immunomicroscopy (IM)

Assay-dependent

-

Miscellaneous PubMed (Misc)

-

View 11 publications 11 publications

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse

Host/Isotype

Rat / IgG2b

Class

Monoclonal

Type

Antibody

Clone

CI:A3-1

Immunogen

Thioglycollate stimulated peritoneal macrophages from C57/BL mice

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2277854

Product Specific Information

Positive control of mouse macrophages suggested. MA1-91124 detects a band of approximately 160 kDa due to the protein being heavily glycosylated, predicted molecular weight of precursor protein is 102 kDa.

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

This antibody recognizes murine F4/80 antigen which shares 68% overall amino acid identity with human EGF module-containing mucin-like hormone receptor 1 (EMR1).

Target Information

F4/80 antigen is a 160 kDa cell surface glycoprotein that is a member of the EGF TM7 family of proteins and shares 68% overall amino acid identity with human EGF module containing mucin like hormone receptor 1 (EMR1). Expression of F4/80 is heterogeneous and is reported to vary during macrophage maturation and activation. The F4/80 antigen is expressed on a wide range of mature tissue macrophages including Kupffer cells, Langerhans, microglia, macrophages located in the gut lamina propria, peritoneal cavity, lung, thymus, bone marrow stroma and macrophages in the red pulp of the spleen. F4/80 expression has also been reported on a subpopulation of dendritic cells but is absent from macrophages located in T cell areas of the spleen and lymph node. The ligands and biological functions of the F4/80 antigen have not yet been determined but recent studies suggest a role for F4/80 in the generation of efferent CD8+ve regulatory T cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.