Thermo Fisher Scientific N-cadherin Monoclonal Antibody (CH-19)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500

View 4 publications 4 publications

Immunohistochemistry (IHC)

-

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:500

-

Immunocytochemistry (ICC/IF)

1:500

View 1 publication 1 publication

Product Specifications

Species Reactivity

Bovine, Dog, Chicken, Cat, Guinea pig, Goat, Hamster, Human, Mouse, Sheep, Pig, Rabbit, Reptile, Rat

Published species

Horse, Mouse

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

CH-19

Immunogen

synthetic peptide DYDYLNDWGPRFKKLADMYGGGDD conjugated to KLH, corresponding to amino acids 889-912 of Chicken N-Cadherin (cdh2). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:N-cadherin,uniProtId:P10288-1,ncbiNodeId:9031,antigenRange:889-912,antigenLength:912,antigenImageFileName:MA1-91128_N-cadherin_P10288-1_House_mouse.svg,antigenImageFileNamePDP:MA1-91128_N-cadherin_P10288-1_House_mouse_PDP.jpeg,sortOrder:3}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

6.1 mg/mL

Storage buffer

Ascites

Contains

15mM sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_1958717

Product Specific Information

Recommended positive controls: MDBK.

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

N-cadherin is a 140 kDa protein belonging to a family of transmembrane molecules that mediate calcium-dependent intercellular adhesion. Cadherins are involved in controlling morphogenetic movements during development and regulate cell surface adhesion through homotypic adhesion with the same cadherin species. N-cadherin`s function is dependent on its association with the actin-cytoskeleton and is mediated through interactions between the C-terminal region of N-cadherin and the cytoplasmic catenin proteins. The stability of this association is regulated by phosphorylation and dephosphorylation of beta-catenin. Further, N-cadherin is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. The protein functions during gastrulation and is required for establishment of left-right asymmetry.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.