Thermo Fisher Scientific N-cadherin Monoclonal Antibody (CH-19)
N-cadherin 단백질을 인식하는 CH-19 클론의 mouse monoclonal 항체. Western blot, IHC, ICC/IF 등 다양한 응용에 사용 가능. 다종 교차 반응성을 가지며, 6.1 mg/mL 농도의 액상 형태로 제공. 단기 4°C, 장기 -20°C 보관 권장.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500 | View 4 publications |
| Immunohistochemistry (IHC) | - | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:500 | - |
| Immunocytochemistry (ICC/IF) | 1:500 | View 1 publication |
Product Specifications
| Item | Description |
|---|---|
| Species Reactivity | Bovine, Dog, Chicken, Cat, Guinea pig, Goat, Hamster, Human, Mouse, Sheep, Pig, Rabbit, Reptile, Rat |
| Published Species | Horse, Mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | CH-19 |
| Immunogen | Synthetic peptide DYDYLNDWGPRFKKLADMYGGGDD conjugated to KLH, corresponding to amino acids 889–912 of Chicken N-Cadherin (cdh2). |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 6.1 mg/mL |
| Storage Buffer | Ascites |
| Contains | 15 mM sodium azide |
| Storage Conditions | Store at 4°C (short term). For long term, store at -20°C. Avoid freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_1958717 |
Product Specific Information
- Recommended positive control: MDBK
- Store product as a concentrated solution.
- Centrifuge briefly prior to opening the vial.
Target Information
N-cadherin is a 140 kDa protein belonging to a family of transmembrane molecules that mediate calcium-dependent intercellular adhesion. Cadherins are involved in controlling morphogenetic movements during development and regulate cell surface adhesion through homotypic adhesion with the same cadherin species.
N-cadherin’s function is dependent on its association with the actin cytoskeleton and is mediated through interactions between the C-terminal region of N-cadherin and the cytoplasmic catenin proteins. The stability of this association is regulated by phosphorylation and dephosphorylation of beta-catenin.
Further, N-cadherin is a calcium-dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region, and a highly conserved cytoplasmic tail. The protein functions during gastrulation and is required for establishment of left-right asymmetry.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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배송/결제/교환/반품 안내
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