Thermo Fisher Scientific CD279 (PD-1) Monoclonal Antibody (J43), FITC, eBioscience

Thermo Fisher Scientific CD279 (PD-1) Monoclonal Antibody (J43), FITC, eBioscience

Applications and Tested Dilution

Product Specifications

Available Formats

• Unconjugated
• APC
• APC-eFluor 780
• Biotin
• Brilliant UV 395 / 563 / 615 / 661 / 737 / 805
• Brilliant Violet 480 / 605 / 650 / 786
• eFluor 450 / 506
• Functional Grade
• PE / PE-Cyanine7 / PE-eFluor 610
• PerCP-eFluor 710
• Super Bright 436 / 600 / 645 / 702 / 780
• Request custom conjugation

Product Specific Information

The J43 monoclonal antibody reacts with mouse PD-1 (programmed death-1), a 55 kDa member of the Ig superfamily. PD-1 contains the immunoreceptor tyrosine-based inhibitory motif (ITIM) and plays a key role in peripheral tolerance and autoimmune disease in mice. PD-1 is expressed mainly on activated T and B lymphocytes.
Two novel B7 Family members, PD-L1 (B7-H1) and PD-L2 (B7-DC), are identified as PD-1 ligands. J43 inhibits the binding of mouse PD-L1-Ig and PD-L2-Ig to PD-1/BHK transfected cells. In vivo administration of J43 enhances contact hypersensitivity and exacerbates acute GVHD, EAE, and NOD diabetes similar to PD-1-deficient cells.

Applications Reported: Flow cytometric analysis
Applications Tested: Flow cytometric analysis of Con-A activated mouse splenocytes (≤1 µg/test)
Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser
Filtration: 0.2 µm post-manufacturing filtered

Target Information

PD-1 (PDCD-1) mediates inhibitory signals in activated T cells when bound to ligands PD-L1 or PD-L2 on antigen-presenting cells. These interactions suppress immune activation in the absence of danger signals such as LPS. PD-1-deficient mice exhibit hyperactivated immune responses and autoimmune disease. Despite its predicted molecular weight, PD-1 often migrates higher in SDS-PAGE.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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