Thermo Fisher Scientific CD279 (PD-1) Monoclonal Antibody (J43), FITC, eBioscience
Mouse PD-1 단백질을 인식하는 J43 클론의 FITC 결합 단클론 항체로, Flow Cytometry 분석에 최적화됨. 488 nm Blue Laser에 적합하며, 0.5 mg/mL 농도의 액상 형태로 제공. 면역관용 및 자가면역 연구용으로 활용 가능.
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Thermo Fisher Scientific CD279 (PD-1) Monoclonal Antibody (J43), FITC, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | View 1 publication |
| Immunohistochemistry (Frozen) (IHC (F)) | - | View 1 publication |
| Immunocytochemistry (ICC/IF) | - | View 1 publication |
| Flow Cytometry (Flow) | 1 µg/test | View 70 publications |
| Neutralization (Neu) | - | View 1 publication |
| Functional Assay | - | View 1 publication |
| In vitro Assay (IV) | - | View 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Human, Mouse |
| Host / Isotype | Armenian hamster / IgG |
| Recommended Isotype Control | Armenian Hamster IgG Isotype Control (eBio299Arm), FITC, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | J43 |
| Conjugate | FITC |
| Excitation / Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_465471 |
Available Formats
- Unconjugated
- APC
- APC-eFluor 780
- Biotin
- Brilliant UV 395 / 563 / 615 / 661 / 737 / 805
- Brilliant Violet 480 / 605 / 650 / 786
- eFluor 450 / 506
- Functional Grade
- PE / PE-Cyanine7 / PE-eFluor 610
- PerCP-eFluor 710
- Super Bright 436 / 600 / 645 / 702 / 780
- Request custom conjugation
Product Specific Information
The J43 monoclonal antibody reacts with mouse PD-1 (programmed death-1), a 55 kDa member of the Ig superfamily. PD-1 contains the immunoreceptor tyrosine-based inhibitory motif (ITIM) and plays a key role in peripheral tolerance and autoimmune disease in mice. PD-1 is expressed mainly on activated T and B lymphocytes.
Two novel B7 Family members, PD-L1 (B7-H1) and PD-L2 (B7-DC), are identified as PD-1 ligands. J43 inhibits the binding of mouse PD-L1-Ig and PD-L2-Ig to PD-1/BHK transfected cells. In vivo administration of J43 enhances contact hypersensitivity and exacerbates acute GVHD, EAE, and NOD diabetes similar to PD-1-deficient cells.
Applications Reported: Flow cytometric analysis
Applications Tested: Flow cytometric analysis of Con-A activated mouse splenocytes (≤1 µg/test)
Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser
Filtration: 0.2 µm post-manufacturing filtered
Target Information
PD-1 (PDCD-1) mediates inhibitory signals in activated T cells when bound to ligands PD-L1 or PD-L2 on antigen-presenting cells. These interactions suppress immune activation in the absence of danger signals such as LPS. PD-1-deficient mice exhibit hyperactivated immune responses and autoimmune disease. Despite its predicted molecular weight, PD-1 often migrates higher in SDS-PAGE.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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