Thermo Fisher Scientific Brachyury Monoclonal Antibody (1H9A2)
Brachyury 단백질(T)을 인식하는 mouse monoclonal antibody로, Human 시료에 반응합니다. WB, IHC, Flow Cytometry, ELISA에 사용 가능하며, 단백질 G로 정제된 1 mg/mL 액상 형태입니다. 단기 4°C, 장기 -20°C 보관 권장.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:1,000 |
| Flow Cytometry (Flow) | 1:200–1:400 |
| ELISA | 1:10,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 1H9A2 |
| Immunogen | Purified recombinant fragment of human T (amino acids 257–309) expressed in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term, store at -20°C. Avoid freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2538656 |
Product Specific Information
MA5-17185 targets Brachyury protein T in indirect ELISA, FACS, IHC, and WB applications, showing reactivity with Human samples.
The immunogen is a purified recombinant fragment of human T (amino acids 257–309) expressed in E. coli.
This antibody detects Brachyury protein T, which has a predicted molecular weight of approximately 47.4 kDa.
Target Information
The protein encoded by the Brachyury (T) gene is an embryonic nuclear transcription factor that binds to a specific DNA element, the palindromic T-site.
It binds through its N-terminal T-box region and regulates transcription of genes required for mesoderm formation and differentiation.
The protein is localized to notochord-derived cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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