Thermo Fisher Scientific Human Nesfatin-1 Recombinant Protein, PeproTech

Applications

Tested Dilution

Publications

Functional Assay (Functional)

Assay-dependent

-

Product Specifications

Species

Human

Expression System

E. coli

Amino acid sequence

VPIDIDKTKV QNIHPVESAK IEPPDTGLYY DEYLKQVIDV LETDKHFREK LQKADIEEIK SGRLSKELDL VSHHVRTKLD EL

Molecular weight

9.7 kDa

Class

Recombinant

Type

Protein

Purity

≥ 98% by SDS-PAGE gel and HPLC analyses.

Endotoxin concentration

<1 EU/µg

Activity

Determined by an in vivo assay using healthy wild type male mice (C57BL/6J). Mice were treated via intraperitoneal injection once at a dose of 4ug Nesfatin-1/gm of body weight. Significant effects on body weight and food consumption were observed relative to saline-treated controls.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Purification

purified

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient

Product Specific Information

300-67-1MG will be provided as 10 x 100 µg (300-67-100UG).

300-67-200UG will be provided as 2 x 100 µg (300-67-100UG).

300-67-500UG will be provided as 5 x 100 µg (300-67-100UG).

Recombinant Human Nesfatin-1 is a 9.7 kDa protein containing 82 amino acid residues.

This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot-specific Certificate of Analysis

Target Information

NUCB2 was initially identified as calcium-binding protein EF hand motif-containing protein that could bind with Necdin, a growth suppressor expressed primarily in postmitotic neurons. NUC2 is a secreted protein that is cleaved into three major peptide products, Nesfatin-1, Nesfatin-2, and Nesfatin-3, of which Nesfatin-1 has been found to cause the suppression of food intake in a leptin-independent manner. Other studies have suggested that NUCB2/Nesfatin-1 may also play roles in energy homeostasis and closely related neuroendocrine functions.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.