Thermo Fisher Scientific CDK3 Monoclonal Antibody (3C12)
CDK3 단백질을 인식하는 Mouse IgG1 단클론 항체. Western blot, IHC, ELISA에 사용 가능. 인간 CDK3에 반응하며, GST 태그 결합 재조합 단백질을 면역원으로 사용. 연구용으로만 제공.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–5 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 3 µg/mL |
| ELISA | 0.1 ng/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 3C12 |
| Immunogen | CDK3 (NP_001249, 206 a.a. to approximately 305 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 kDa. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | See Label |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Sequence of this protein is as follows:
DSEIDQLFRI FRMLGTPSED TWPGVTQLPD YKGSFPKWTR KGLEEIVPNL EPEGRDLLMQ LLQYDPSQRI TAKTALAHPY FSSPEPSPAA RQYVLQRFRH
Target Information
CDK3/Cyclin E1 is a member of the cyclin-dependent protein kinase family and promotes entry into S phase, in part by activating members of the E2F family of transcription factors. CDK3 can also associate with cyclin C and phosphorylates the retinoblastoma 1 protein to promote exit from G0.
The CDK3 gene has been mapped to chromosomal location 17q22–qter, telomeric to the BRCA1 gene by somatic cell hybrid analysis.
A single point mutation in the CDK3 gene from several Mus musculus strains commonly used in the laboratory has been reported.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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