Thermo Fisher Scientific TRIF Polyclonal Antibody
TRIF 단백질을 인식하는 Rabbit Polyclonal Antibody로, Western blot, IHC, ICC, IP에 사용 가능. 인간 시료에 반응하며, PBS buffer에 0.02% sodium azide 함유. 2–8°C 보관, 장기 보관은 -20°C 권장. 연구용으로만 사용.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1–2 µg/mL | View 1 publication |
| Immunohistochemistry (IHC) | 10 µg/mL | - |
| Immunocytochemistry (ICC/IF) | 10 µg/mL | - |
| Immunoprecipitation (IP) | - | View 1 publication |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Not Applicable |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A peptide corresponding to 14 amino acids near the C-terminus of human TRIF. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Ion-exchange chromatography |
| Storage Buffer | PBS |
| Contains | 0.02% sodium azide |
| Storage Conditions | Maintain refrigerated at 2–8°C for up to 3 months. For long-term storage, store at -20°C. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_11154785 |
Product Specific Information
- Suggested positive control: human lung tissue lysate.
- PA5-20030 can be used with blocking peptide PEP-0149.
Target Information
TRIF is involved in innate immunity against invading pathogens. It acts as an adapter used by TLR3 and TLR4 (through TICAM2) to mediate NF-kappa-B and interferon-regulatory factor (IRF) activation, and to induce apoptosis. Ligand binding to these receptors results in TRIF recruitment through its TIR domain. Distinct protein-interaction motifs allow recruitment of effector proteins TBK1, TRAF6, and RIPK1, leading to activation of transcription factors IRF3, IRF7, NF-kappa-B, and FADD respectively.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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