Thermo Fisher Scientific DTYMK Monoclonal Antibody (OTI1H4), TrueMAB
DTYMK 단백질을 인식하는 Mouse monoclonal antibody로 Western blot, ICC/IF, Flow cytometry에 적합. Human 시료 반응성. Lyophilized 형태로 제공되며, 재구성 후 1 mg/mL 농도. DNA 합성 관련 효소 연구에 유용.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI1H4 |
| Immunogen | Full length human recombinant protein of human DTYMK produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL of distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step (recommended: Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
Thymidylate kinase (DTYMK) catalyzes the phosphorylation of dTMP to form dTDP in the dTTP synthesis pathway essential for DNA synthesis.
DTYMK is a key intermediate enzyme in the pathway of pyrimidine analog drugs such as 5-Fluorouracil (5-FU), used in chemotherapy for several solid tumors.
The human DTYMK gene was identified through functional complementation of a Saccharomyces cerevisiae cell cycle mutant (cdc8).
DTYMK mRNA and enzyme activity are regulated by cell cycle and growth pathways.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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