Thermo Fisher Scientific SP1 Polyclonal Antibody
SP1 단백질을 인식하는 Rabbit Polyclonal Antibody로, WB, IHC, ICC/IF, ELISA 등 다양한 응용에 적합합니다. Human, Mouse, Rat 시료 반응성이 있으며, 고순도 Affinity Chromatography 정제. PBS/glycerol buffer에 보관하며 연구용으로만 사용됩니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| ELISA | 1 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 420–620 of human SP1 (NP_612482.2) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.664 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.09% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2913858 |
Product Specific Information
Positive test controls include Mouse liver, Mouse heart, and Rat liver.
The target is typically localized in the Cytoplasm and Nucleus.
Immunogen sequence:
LSGQTFTTQA ISQETLQNLQ LQAVPNSGPI IIRTPTVGPN GQVSWQTLQL QNLQVQNPQA QTITLAPMQG VSLGQTSSSN TTLTPIASAA SIPAGTVTVN AAQLSSMPGL QTINLSALGT SGIQVHPIQG LPLAIANAPG DHGAQLGLHG AGGDGIHDDT AGGEEGENSP DAQPQAGRRT RREACTCPYC KDSEGRGSGD P
Target Information
SP1 is a transcription factor that can activate or repress transcription in response to physiological and pathological stimuli. It binds with high affinity to GC-rich motifs and regulates the expression of numerous genes involved in cell growth, apoptosis, differentiation, and immune responses.
SP1 activity is modulated by post-translational modifications such as phosphorylation, sumoylation, proteolytic cleavage, glycosylation, and acetylation.
It also binds to the PDGFR-alpha G-box promoter and may play a role in modulating cellular responses to DNA damage.
SP1 is implicated in chromatin remodeling and recruitment of SMARCA4/BRG1 on the c-FOS promoter, and is essential for FE65 gene regulation.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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