Thermo Fisher Scientific MTH1 Recombinant Rabbit Monoclonal Antibody (23GB4060)

Applications

Western Blot (WB)

• Tested Dilution: 1:1,000–1:5,000

Immunocytochemistry (ICC/IF)

• Tested Dilution: 1:1,000

Flow Cytometry (Flow)

• Tested Dilution: 1:2,000

Product Specifications

Target Information

Oxygen radicals damage chromosomal DNA causing cell death and inducing mutations. Among the various classes of DNA damage caused by oxygen radicals, an oxidized form of guanine base (8-oxoguanine) appears to be important as it can pair with cytosine and adenine, leading to G:C to T:A transversion mutations. A significant amount of 8-OxoG is formed in the chromosomal DNA of mammalian cells, with most damaged nucleotides excised and excreted in the urine.

In addition to 8-oxoG present in oxidatively damaged DNA, 8-oxo-deoxyguanosine (8-oxo-dGTP) is formed in the nucleotide pool during normal metabolism and oxidative stress. The 8-oxo-dGTP nucleotide can be incorporated into DNA during polymerization, causing mispairing unless repaired. MTH converts 8-oxo-dGTP to its monophosphate form, preventing misincorporation into DNA. MTH also recognizes 8-oxo-rGTP, which could incorporate into RNA during transcription and lead to missense or nonsense protein production.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.