Thermo Fisher Scientific APEX1 Monoclonal Antibody (OTI1H9), TrueMAB

Applications

Western Blot (WB)

• Tested Dilution: 1:2,000

Immunohistochemistry (Paraffin) (IHC (P))

• Tested Dilution: 1:150

Product Specifications

Target Information

Mammalian apurinic/apyrimidinic endonuclease (APE/ref-1) is a multifunctional, bipartite enzyme that plays an important role in numerous cellular functions.
APE is responsible for repairing abasic sites in DNA and regulating the redox state of proteins involved in oxidative signaling, transcription factor regulation (Fos, Jun, NF-kB, Myb, HIF-1α, CREB, Pax), cell cycle control (p53), and apoptosis.
Abasic sites arise through spontaneous loss or oxidative DNA damage, chemically initiated hydrolysis, ionizing radiation, UV irradiation, oxidizing agents, and removal of modified bases by DNA glycosylases.
APE expression varies during development and across tissues, with diverse subcellular localization supporting interactions with other cellular proteins beyond nuclear DNA repair.
Regulation of APE by phosphorylation is mediated partly by casein kinase II.
Increases in APE message and protein levels are observed upon reoxygenation of hypoxic cells and in certain malignant tissues, while decreases are associated with apoptosis induction.

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.