Thermo Fisher Scientific Mouse Hematopoietic Lineage Antibody Cocktail, FITC, eBioscience

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

20 µL/test

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Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat

Class

Cocktail

Type

Antibody

Clone

17A2, RA3-6B2, M1/70, TER-119, RB6-8C5

Conjugate

FITC FITC FITC

Excitation/Emission Max

498/517 nm View spectra

Form

Liquid

Concentration

20 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2644066

Product Specific Information

Description: The Mouse Hematopoietic Lineage FITC Cocktails contains FITC-conjugated antibodies that can be used to identify, enrich and/or deplete cells committed to the T, B, NK, myeloid and erythroid lineages based on the expression of cell surface antigens.

This cocktail contains the following antibodies:

Anti-Mouse CD3 (17A2) FITC

Anti-Mouse CD45R (B220) (RA3-6B2) FITC

Anti-Mouse CD11b (M1/70) FITC

Anti-Mouse TER-119 (TER-119) FITC

Anti-Mouse Ly-G6 (Gr-1) (RB6-8C5) FITC

Applications Reported: The Mouse Hematopoietic Lineage FITC Cocktail has been reported for use in flow cytometric analysis.

Applications Tested: The Mouse Hematopoietic Lineage FITC Cocktail has been pre-titrated and tested by flow cytometric analysis of C57Bl/6 bone marrow cells. This can be used at 20 µL per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Target Information

Hematopoietic Lineage antibodies can be used to identify, enrich and/or deplete cells committed to the T, B, NK, myeloid and erythroid lineages based on their expression of cell surface antigens.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.