Thermo Fisher Scientific CD163 Monoclonal Antibody (OTI2C1), TrueMAB

Applications and Tested Dilution

Product Specifications

Product Specific Information

For reconstitution, add 100 µL of distilled water to obtain a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, it is recommended to perform an additional desalting step (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).

Target Information

CD163 (M130 antigen, Ber-Mac3, Ki-M8, SM4) is a 130 kDa membrane glycoprotein belonging to the scavenger receptor cysteine-rich superfamily. It functions as a receptor for the hemoglobin-haptoglobin complex and protects tissues from oxidative damage caused by free hemoglobin. CD163 is involved in iron recycling through endocytosis of hemoglobin/haptoglobin and subsequent heme breakdown.

CD163 is expressed on human monocytes and macrophages, particularly in the late phase of inflammation. It is present on all circulating monocytes and most tissue macrophages, except in mantle zones, germinal centers, interdigitating reticulum cells, and Langerhans cells. Expression is higher in CD16⁺ monocytes and upregulated by IL-10, IL-6, and dexamethasone. LPS and PMA induce shedding of CD163 from the cell surface into plasma or supernatant.

CD163 binds hemoglobin/haptoglobin complexes in a calcium- and pH-dependent manner, showing higher affinity for multimeric haptoglobin (HP1F phenotype) complexes. It also triggers intracellular signaling cascades involving tyrosine kinase-dependent calcium mobilization, inositol triphosphate production, and secretion of IL-6 and CSF1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.