Thermo Fisher Scientific UBE1 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

-

Immunocytochemistry (ICC/IF)

1:50-1:200

-

ELISA (ELISA)

1 µg/mL

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 300-600 of human UBA1 (NP_6950121) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:UBE1,uniProtId:P22314-1,ncbiNodeId:9606,antigenRange:300-600,antigenLength:1058,antigenImageFileName:PA5-88493_UBE1_P22314-1_Rabbit.svg,antigenImageFileNamePDP:PA5-88493_UBE1_P22314-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.57 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.01% thimerosal

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2804952

Product Specific Information

Immunogen sequence: KISFKSLVAS LAEPDFVVTD FAKFSRPAQL HIGFQALHQF CAQHGRPPRP RNEEDAAELV ALAQAVNARA LPAVQQNNLD EDLIRKLAYV AAGDLAPINA FIGGLAAQEV MKACSGKFMP IMQWLYFDAL ECLPEDKEVL TEDKCLQRQN RYDGQVAVFG SDLQEKLGKQ KYFLVGAGAI GCELLKNFAM IGLGCGEGGE IIVTDMDTIE KSNLNRQFLF RPWDVTKLKS DTAAAAVRQM NPHIRVTSHQ NRVGPDTERI YDDDFFQNLD GVANALDNVD ARMYMDRRCV YYRKPLLESG T; Positive Samples: U-251MG, Rat spleen; Cellular Location: Cytoplasm, Mitochondrion, Nucleus, Nucleus

Target Information

UBE1 catalyzes the first step in ubiquitin conjugation to mark cellular proteins for degradation. This gene complements an X-linked mouse temperature-sensitive defect in DNA synthesis, and thus may function in DNA repair. It is part of a gene cluster on chromosome Xp11. 23. Alternative splicing results in 2 transcript variants encoding the same protein, but with different 5` UTR.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.