Thermo Fisher Scientific CD19 Monoclonal Antibody (eBio1D3 (1D3)), Super Bright 436, eBioscience
마우스 CD19를 인식하는 eBio1D3 (1D3) 단클론 항체로, Super Bright 436 형광체로 결합됨. Flow cytometry에 최적화되어 있으며, B세포 발현 분석에 활용. 405 nm 레이저로 여기, 436 nm에서 방출. 4°C 암소 보관, 동결 금지.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | 1 publication |
| Flow Cytometry (Flow) | 0.5 µg/test | 24 publications |
Product Specifications
| 항목 | 내용 |
|---|---|
| Published species | Not Applicable |
| Host / Isotype | Rat / IgG2a, kappa |
| Recommended Isotype Control | Rat IgG2a kappa Isotype Control (eBR2a), Super Bright™ 436, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | eBio1D3 (1D3) |
| Conjugate | Super Bright™ 436 |
| Excitation/Emission Max | 413/431 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Storage conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping conditions | Wet ice |
| RRID | AB_2688106 |
Available Formats
- Unconjugated
- Alexa Fluor 561, 660, 700
- APC, APC-eFluor 780
- Biotin
- Brilliant UV Series (395–805)
- Brilliant Violet Series (421–786)
- eFluor 450, 506, 660
- FITC
- Functional Grade
- NovaFluor Series (Blue, Red, Violet, Yellow)
- PE, PE-Cyanine Series, PE-eFluor 610
- PerCP-Cyanine5.5
- Super Bright Series (436, 600, 645, 702, 780)
- Request custom conjugation
Product Specific Information
Description:
The eBio1D3 (1D3) monoclonal antibody reacts with mouse CD19, a 95 kDa transmembrane glycoprotein expressed on B cells during all developmental stages except plasma cells. Follicular dendritic cells also express CD19. CD19 forms a complex with CD21, CD81, and MHC class II, associating with the BCR to mediate signaling that induces phosphorylation, calcium flux, and proliferation in B cells.
Applications Reported:
Reported for use in flow cytometric analysis.
Applications Tested:
Tested by flow cytometric analysis of mouse splenocytes. Recommended ≤0.5 µg per test (100 µL final volume, 10⁵–10⁸ cells/test). Optimize antibody titration for best performance.
Fluorochrome Information:
Super Bright 436 can be excited with a 405 nm violet laser and emits at 436 nm. Recommended detection with a 450/50 bandpass filter. Ensure instrument compatibility.
Staining Recommendations:
When using multiple Super Bright-conjugated antibodies, use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize nonspecific interactions.
Excitation: 405 nm
Emission: 436 nm
Laser: Violet Laser
Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
Target Information
CD19 is an immunoglobulin superfamily member with two Ig-like domains, expressed on all peripheral B cells. It is also found on certain myeloid leukemia cells. CD19 functions as a key regulator of B cell receptor signaling, lowering the activation threshold for antigen-dependent stimulation. Mutations can cause hypogammaglobulinemia, while overexpression leads to B cell hyperactivity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without authorization.
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