Thermo Fisher Scientific Maltose Binding Protein (MBP) Epitope Tag Polyclonal Antibody
MBP 에피토프 태그 단백질 검출용 폴리클로날 항체로, Western blot, ELISA, IP 등 다양한 응용에 적합합니다. Rabbit IgG 기반 비결합 항체이며, Protein A로 정제되었습니다. MBP 태그 단백질에 특이적이며 비특이적 반응이 적습니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| ELISA | 1:10,000–1:50,000 |
| Immunoprecipitation (IP) | 1:1,000–1:5,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Tag |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | This antibody was purified from whole rabbit serum prepared by repeated immunizations with the MBP epitope tag recombinant protein. |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 0.15M NaCl |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Store vial at 4°C prior to restoration. For extended storage, aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.
This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
This IgG purified antibody is directed against MBP and is useful in determining its presence in various assays.
This polyclonal anti-MBP tag antibody detects over-expressed proteins containing the MBP epitope tag.
To date, this antibody has reacted with all MBP-tagged proteins tested.
In western blotting of bacterial extracts, the antibody does not cross-react with endogenous proteins.
Target Information
The approach of epitope tagging offers an easy and universal strategy for the identification and purification of proteins derived by recombinant DNA technology.
Insertion of a Maltose Binding Protein (MBP) tag creates a stable fusion product that does not interfere with the bioactivity or biodistribution of the MBP-tagged protein.
Cleavage by factor Xa separates MBP from its partner protein.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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