Thermo Fisher Scientific CD25 Monoclonal Antibody (PC61.5), Alexa Fluor 532, eBioscience

Applications

Product Specifications

Additional Formats Available

Unconjugated, Alexa Fluor 488, Alexa Fluor 561, Alexa Fluor 647, Alexa Fluor 660, Alexa Fluor 700, APC, APC-eFluor 780, Biotin, Brilliant UV 563, Brilliant UV 661, Brilliant UV 737, Brilliant UV 805, Brilliant Violet 421, Brilliant Violet 650, Brilliant Violet 711, Brilliant Violet 786, eFluor 450, eFluor 506, FITC, Functional Grade, NovaFluor Blue 585, NovaFluor Blue 610-70S, NovaFluor Blue 660-120S, NovaFluor Blue 760, Pacific Orange, PE, PE-Cyanine5, PE-Cyanine5.5, PE-Cyanine7, PE-eFluor 610, PerCP-Cyanine5.5, PerCP-eFluor 710, Super Bright 436, Super Bright 600, Super Bright 645, Super Bright 702, Super Bright 780.
Custom conjugation available upon request.

Product Specific Information

Description:
The PC61.5 antibody reacts with mouse CD25, the 55 kDa interleukin-2 receptor alpha chain (IL-2Rα). CD25 is expressed by early progenitors of T and B lineage as well as by activated mature T and B lymphocytes. CD25 alone binds IL-2 with low affinity, but forms a high-affinity receptor complex with CD122 (IL-2Rβ) and CD132 (common γ chain). The PC61.5 antibody inhibits IL-2 binding to both high and low affinity IL-2 receptors. CD25 is involved in lymphocyte differentiation and activation/proliferation.

Applications Reported:
Reported for use in flow cytometric analysis.

Applications Tested:
Tested by flow cytometric analysis of mouse splenocytes. Recommended ≤0.25 µg per test (in 100 µL final volume). Cell number may range from 10⁵–10⁸ cells/test. Optimize titration for best performance.

Fluorophore Information:
Alexa Fluor™ 532 is excited with the Green laser (532 nm) and emits at 561 nm. Not compatible with Yellow-Green laser (561 nm). Recommended filter: 560/14 band pass. Ensure instrument compatibility.
Excitation: 532 nm; Emission: 561 nm; Laser: Green Laser.

Target Information

CD25 (IL2RA):
CD25 encodes the interleukin-2 receptor alpha chain, part of a heterotrimeric receptor complex with IL2RB and IL2RG forming the high-affinity IL-2 receptor. IL-2 plays key roles in T and B cell proliferation, differentiation, and regulation of cell death. Mutations in IL2RA are associated with interleukin-2 receptor alpha deficiency and immune dysregulation.

For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.

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