Thermo Fisher Scientific TLE1 Monoclonal Antibody (UMAB253), UltraMAB

Applications

Tested Dilution

Publications

Western Blot (WB)

1:2,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:300

-

Immunocytochemistry (ICC/IF)

1:100

-

ChIP assay (ChIP)

Assay-Dependent

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

UMAB253

Immunogen

Human recombinant protein fragment corresponding to amino acids 180-460 of human TLE1 produced in E.coli. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:TLE1,uniProtId:Q04724-1,ncbiNodeId:9606,antigenRange:180-460,antigenLength:770,antigenImageFileName:UM800143_TLE1_Q04724-1_House_mouse.svg,antigenImageFileNamePDP:UM800143_TLE1_Q04724-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5-1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS with 1% BSA, 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Target Information

Transcriptional corepressor that binds to a number of transcription factors. Inhibits NF-kappa-B-regulated gene expression. Inhibits the transcriptional activation mediated by FOXA2, and by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES. Unusual function as coactivator for ESRRG.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.