Thermo Fisher Scientific ID3 Polyclonal Antibody
Human ID3 단백질에 특이적인 Rabbit Polyclonal Antibody. IHC(P)에서 1:1,000~1:2,500 희석으로 사용 가능. 항원 친화 크로마토그래피로 정제. 4°C 단기 보관, 장기 보관은 -20°C 권장. 연구용으로만 사용.
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Applications
Immunohistochemistry (Paraffin) [IHC (P)]
- Tested Dilution: 1:1,000–1:2,500
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein corresponding to Human ID3. Recombinant protein control fragment (Product #RP-94113) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2642621 |
Product Specific Information
- Immunogen sequence:
VPRGTQLSQV EILQRVIDYI LDLQVVLAEP APGPPDGPHL PIQTAELAPE LVISND - Sequence identity:
Highest antigen sequence identity to the following orthologs:- Mouse: 96%
- Rat: 96%
Target Information
Members of the Id family of basic helix-loop-helix (bHLH) proteins include ID1, ID2, ID3, and ID4. These proteins are ubiquitously expressed and dimerize with class A and B HLH proteins. Due to the absence of the basic region, the resulting heterodimers cannot bind DNA, thus functioning as negative regulators of DNA binding by bHLH proteins.
ID1 inhibits DNA binding of E12 and Myo D, thereby suppressing muscle-specific gene expression. During muscle cell differentiation, ID protein levels decrease, allowing E12/E47 to form heterodimers with Myo D and myogenin, which activate myogenic differentiation. Expression of ID proteins is dependent on growth factor activation, and reduction of ID mRNA levels delays reentry of arrested cells into the cell cycle after growth factor stimulation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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