Thermo Fisher Scientific HLA-DQB1 Polyclonal Antibody
Human HLA-DQB1 단백질을 인식하는 Rabbit Polyclonal Antibody로, Western blot 및 IHC(P) 검증 완료. 항원 친화 크로마토그래피로 정제되었으며, PBS/glycerol buffer에 보관. 면역펩티도믹스 연구 및 항원 제시 관련 연구에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 0.04–0.4 µg/mL | – |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 | – |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein corresponding to Human HLA-DQB1. Recombinant protein control fragment (Product #RP-90524) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.05 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C (short term). For long-term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2789645 |
Product Specific Information
Immunogen sequence:
RPDAEYWNSQ KEVLEGTRAE LDTVCRHNYE VAFRGILQRR VEPTVTISPS RTEALNHHNL LVCSVT
Target Information
HLA-DRB1 belongs to the HLA class II beta chain paralogs. The class II molecule is a heterodimer consisting of an alpha (DRA) and a beta chain (DRB), both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen-presenting cells (APC: B lymphocytes, dendritic cells, macrophages).
The beta chain is approximately 26–28 kDa and encoded by 6 exons:
- Exon 1: leader peptide
- Exons 2–3: extracellular domains
- Exon 4: transmembrane domain
- Exon 5: cytoplasmic tail
Within the DR molecule, the beta chain contains all the polymorphisms specifying peptide-binding specificities. Hundreds of DRB1 alleles have been described, and typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. DRB1 is expressed at a level five times higher than its paralogs DRB3, DRB4, and DRB5. DRB1 is present in all individuals. Allelic variants of DRB1 are linked with either none or one of the genes DRB3, DRB4, and DRB5. There are four related pseudogenes: DRB2, DRB6, DRB7, DRB8, and DRB9.
HLA and MHC antibodies play a significant role in Immunopeptidomics, facilitating the identification and characterization of neoantigens through high-performance liquid chromatography coupled to tandem mass spectrometry.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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