Thermo Fisher Scientific Lambda Light Chain (B-Cell Marker) Monoclonal Antibody (HP6054)
B세포 마커인 Lambda Light Chain을 인식하는 Thermo Fisher Scientific의 단클론 항체. Western blot, IHC, ICC, Flow cytometry 등 다양한 응용 가능. 인간 시료 반응성, Mouse IgG2a, kappa 아이소타입. 단백질 A/G 정제, PBS buffer, 방부제 없음.
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Applications and Tested Dilutions
| Application | Tested Dilution | Notes |
|---|---|---|
| Western Blot (WB) | 1–2 µg/mL | |
| Immunohistochemistry (Paraffin) (IHC-P) | Assay-dependent | |
| Immunohistochemistry (PFA fixed) (IHC-PFA) | 1–2 µg/mL | |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL | |
| Flow Cytometry (Flow) | 1–2 µg/10⁶ cells | |
| Immunomicroscopy (IM) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | HP6054 |
| Immunogen | Purified human IgG myeloma proteins coupled to polyaminostyrene microbeads |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4 |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Additional Formats
- CF 488 format available
- Custom conjugation available upon request
Target Information
Immunoglobulin classes share a basic four-polypeptide chain structure of two heavy and two light chains (kappa, lambda; both ~22.5 kDa). Kappa and lambda chains consist of variable and constant regions, distinguishable by antigenic properties of the constant region. The kappa to lambda ratio is approximately 70:30. Antibodies to the kappa light chain are useful for identifying leukemias, plasmacytomas, and certain non-Hodgkin’s lymphomas. Demonstration of clonality in lymphoid infiltrates indicates malignancy.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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