Thermo Fisher Scientific CD171 Monoclonal Antibody (eBio5G3 (5G3)), Super Bright 600, eBioscience
CD171(L1CAM)을 인식하는 마우스 단클론 항체로, Super Bright 600 형광체로 표지되어 있습니다. 인간 시료에 반응하며 Flow Cytometry에 적합합니다. 신경세포 부착 및 종양 관련 연구에 유용하며, 광민감성이 있어 어둠에서 보관해야 합니다.
- 카탈로그번호
- 63-1719-4x (2개 옵션)
- 판매단위
- pk
카탈로그
2개 옵션 · 카탈로그 번호를 클릭하면 복사됩니다Thermo Fisher Scientific · Thermo Fisher Scientific CD171 Monoclonal Antibody (eBio5G3 (5G3)), Super Bright 600, eBioscience
Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | - | View 4 publications |
| Immunohistochemistry (IHC) | - | View 1 publication |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | - | View 1 publication |
| Flow Cytometry (Flow) | 5 µL (0.25 µg)/test | View 2 publications |
| Immunoprecipitation (IP) | - | View 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Published Species | Not Applicable |
| Host / Isotype | Mouse / IgG2a |
| Recommended Isotype Control | Mouse IgG2a kappa Isotype Control (eBM2a), Super Bright™ 600, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | eBio5G3 (5G3) |
| Conjugate | Super Bright™ 600 |
| Excitation/Emission Max | 414 / 601 nm |
| Form | Liquid |
| Concentration | 5 µL/Test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2762553 |
Additional Formats
Product Specific Information
Description:
The monoclonal antibody eBio5G3 recognizes CD171 (L1CAM), a neural cell adhesion molecule belonging to the Ig superfamily. CD171 mediates cell-cell interactions in neuronal myelination, neurite outgrowth, and regeneration. It is expressed on monocytes, dendritic cells, and certain tumor cell lines. The epitope recognized by eBio5G3 is located in the amino-terminal Ig-like domain.
Applications Reported:
Reported for use in flow cytometric analysis.
Applications Tested:
Pre-titrated and tested by flow cytometric analysis of Panc-1 tumor cell line. Recommended use: 5 µL (0.25 µg) per test in a final volume of 100 µL. Suitable for 10⁵–10⁸ cells/test.
Fluorochrome Information:
Super Bright 600 is a tandem dye excited with the violet laser (405 nm) and emits at 600 nm. Recommended detection filter: 610/20 bandpass. Ensure instrument compatibility.
Usage Recommendations:
- Use Super Bright Complete Staining Buffer (Product # SB-4401) when combining multiple Super Bright dyes.
- Protect from light to prevent photo-induced oxidation.
- Samples can be fixed using IC Fixation Buffer (Product # 00-8222) or 1-step Fix/Lyse Solution (Product # 00-5333) and stored for up to 3 days at 2–8°C.
Excitation: 405 nm
Emission: 600 nm
Laser: Violet Laser
Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
Target Information
L1CAM/CD171 is an axonal glycoprotein of the immunoglobulin superfamily, consisting of multiple Ig-like and fibronectin-like domains. It plays a critical role in neuronal migration, differentiation, and development. Mutations in this gene are associated with CRASH syndrome (corpus callosum hypoplasia, retardation, aphasia, spastic paraplegia, and hydrocephalus). Alternative splicing of a neuron-specific exon may have functional significance.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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