
Thermo Fisher Scientific Fibronectin Recombinant Rabbit Monoclonal Antibody (014)
Human Fibronectin 인식용 재조합 토끼 단클론 항체로, 높은 특이성과 재현성을 제공합니다. Western blot, ICC/IF, ELISA, IP 등 다양한 응용에 적합합니다. 보존제가 없는 형태로 제공되며, 단기 4°C, 장기 -20°C 보관 권장.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| ELISA | 1:5,000–1:10,000 |
| Immunoprecipitation (IP) | 1–4 µL/mg of lysate |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 014 |
| Immunogen | Recombinant Human Fibronectin Fragment 2 protein (Ser607–Pro1265) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS |
| Contains | No preservative |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2785172 |
Product Specific Information
This product is preservative-free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%–0.1%).
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The antibody DNA sequences from immunoreactive rabbits are cloned and screened to select optimal candidates.
Advantages include:
- High specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulation
- Broad immunoreactivity due to the large rabbit immune repertoire
This antibody specifically recognizes Human Fibronectin Fragment 2.
Target Information
Fibronectin is a disulfide-bonded dimer (230–250 kDa) found in the extracellular matrix of connective tissues and vessels. It exists as insoluble, cross-linked polymers and as soluble plasma proteins.
Fibronectin plays critical roles in:
- Cell adhesion and migration
- Embryogenesis and wound healing
- Blood coagulation and host defense
- Cancer metastasis
Fibronectin expression is elevated in non-small cell lung carcinoma, where it enhances tumorigenicity and resistance to apoptosis induced by chemotherapeutic agents.
The gene encoding Fibronectin undergoes alternative splicing, producing up to 20 transcript variants, though not all full-length forms are characterized.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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