Thermo Fisher Scientific FSTL1 Recombinant Rabbit Monoclonal Antibody (026)

Applications and Tested Dilution

Product Specifications

Product Specific Information

This product is preservative-free. It is recommended to add sodium azide to avoid contamination (final concentration 0.05%–0.1%).

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The antibody DNA sequences from immunoreactive rabbits are cloned, and individual clones are screened to select the best candidates for production.

Advantages of recombinant rabbit monoclonal antibodies include:

• Better specificity and sensitivity
• Lot-to-lot consistency
• Animal origin-free formulations
• Broader immunoreactivity due to the large rabbit immune repertoire

This antibody has specificity for Human FSTL1.

Target Information

Follistatin-like protein 1 (FSTL1) is a widely expressed extracellular glycoprotein, belonging to the osteonectin (BM-40/SPARC) family. It contains both a follistatin-like and an extracellular calcium-binding domain.

Initially identified as a TGF-beta-inducible protein in a mouse osteoblast cell line, FSTL1 is involved in diverse cellular processes including:

• Regulation of proliferation, differentiation, apoptosis, and migration
• Embryonic development, inflammatory response, angiogenesis, tumorigenesis, and immune disease pathogenesis

FSTL1 is highly conserved across mammalian species and widely expressed in human tissues. It can be upregulated by innate immune mediators such as TLR4 agonists and IL-1β via NFκB pathways, leading to the expression of pro-inflammatory cytokines (TNF-α, IL-1β, IL-6, IL-8).

While mesenchymal cells produce FSTL1, hematopoietic cells typically do not under normal conditions. However, macrophages and monocytes can uptake FSTL1 at inflammation sites, inducing caspase-1 activation and subsequent IL-1β processing.

Clinical relevance:

• Overexpression contributes to immune diseases such as rheumatoid arthritis (RA) and osteoarthritis (OA).
• Reduced serum levels are associated with ovarian and endometrial cancer progression, affecting cell proliferation, migration, and invasion.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.