Thermo Fisher Scientific SKP1 Monoclonal Antibody (4E11)
SKP1 단백질을 검출하기 위한 Thermo Fisher Scientific의 마우스 단클론 항체입니다. Western blot, IHC, ICC, Flow Cytometry, ELISA 등 다양한 응용에 사용 가능합니다. 인간, 마우스, 비인간 영장류에 반응하며, 19kDa 단백질을 인식합니다. 연구용으로만 사용됩니다.
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Applications and Tested Dilutions
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:2,000 | View 3 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:1,000 | - |
| Immunocytochemistry (ICC/IF) | 1:200–1:1,000 | - |
| Flow Cytometry (Flow) | 1:200–1:400 | - |
| ELISA | 1:10,000 | - |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Non-human primate |
| Published Species | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 4E11 |
| Immunogen | Purified recombinant fragment of human SKP1 expressed in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | Not determined |
| Storage Buffer | Ascites |
| Contains | 0.03% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_11153724 |
Product Specific Information
MA5-15928 targets SKP1 in indirect ELISA, FACS, IF, IHC, and WB applications, showing reactivity with human samples.
The immunogen is a purified recombinant fragment of human SKP1 expressed in E. coli.
MA5-15928 detects SKP1, which has a predicted molecular weight of approximately 19 kDa.
Target Information
This gene encodes a component of SCF complexes, which are composed of this protein, cullin 1, a ring-box protein, and one member of the F-box family of proteins.
This protein binds directly to the F-box motif found in F-box proteins. SCF complexes are involved in the regulated ubiquitination of specific protein substrates, targeting them for degradation by the proteasome.
Specific F-box proteins recognize different target proteins, and many SCF substrates have been identified, including regulators of cell cycle progression and development.
Studies have also characterized this protein as an RNA polymerase II elongation factor.
Alternative splicing of this gene results in two transcript variants. A related pseudogene has been identified on chromosome 7.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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